The chicken c-erbA alpha-product induces expression of thyroid hormone-responsive genes in 3,5,3'-triiodothyronine receptor-deficient rat hepatoma cells.

TY - JOUR

T1 - The chicken c-erbA alpha-product induces expression of thyroid hormone-responsive genes in 3,5,3'-triiodothyronine receptor-deficient rat hepatoma cells.

AU - Muñoz, A

AU - Höppner, W

AU - Sap, J

AU - Brady, G

AU - Nordström, K

AU - Seitz, H J

AU - Vennström, B

N1 - Keywords: Animals; Base Sequence; Chickens; Cycloheximide; Gene Expression; Genes; Genomic Library; Liver Neoplasms, Experimental; Malate Dehydrogenase; Molecular Sequence Data; Phosphoenolpyruvate Carboxykinase (GTP); Promoter Regions (Genetics); Proto-Oncogene Proteins; Rats; Receptors, Thyroid Hormone; Retroviridae Infections; Sodium-Potassium-Exchanging ATPase; Thyroid Hormones; Transcription, Genetic; Triiodothyronine

PY - 1990

Y1 - 1990

N2 - To determine the capacity of the chicken c-erbA (cTR-alpha) gene product in regulating expression of known thyroid hormone-responsive genes, both the cTR-alpha and the viral v-erbA genes were expressed in FAO cells, a rat hepatoma cell line defective for functional thyroid hormone receptors. Upon nuclear expression of the cTR-alpha protein the cells become responsive to thyroid hormone, as detected by expression of a number of genes (malic enzyme, phosphoenolpyruvate carboxykinase, and Na+/K(+)-ATPase) reported to be indirectly induced by the hormone in vivo. In addition, our data show that the c-erbA product directly activates the Moloney murine leukemia virus promoter in a ligand-dependent manner. The data show that the chicken c-erbA-alpha protein can modulate the expression of rat genes under either direct or indirect control by thyroid hormone.

AB - To determine the capacity of the chicken c-erbA (cTR-alpha) gene product in regulating expression of known thyroid hormone-responsive genes, both the cTR-alpha and the viral v-erbA genes were expressed in FAO cells, a rat hepatoma cell line defective for functional thyroid hormone receptors. Upon nuclear expression of the cTR-alpha protein the cells become responsive to thyroid hormone, as detected by expression of a number of genes (malic enzyme, phosphoenolpyruvate carboxykinase, and Na+/K(+)-ATPase) reported to be indirectly induced by the hormone in vivo. In addition, our data show that the c-erbA product directly activates the Moloney murine leukemia virus promoter in a ligand-dependent manner. The data show that the chicken c-erbA-alpha protein can modulate the expression of rat genes under either direct or indirect control by thyroid hormone.

M3 - Journal article

C2 - 2158623

SN - 0888-8809

VL - 4

SP - 312

EP - 320

JO - Molecular Endocrinology

JF - Molecular Endocrinology

IS - 2

ER -