TY - JOUR
T1 - The application of the CRISPR-Cas9 genome editing machinery in food and agricultural science
T2 - Current status, future perspectives, and associated challenges
AU - Eş, Ismail
AU - Gavahian, Mohsen
AU - Marti-Quijal, Francisco J.
AU - Lorenzo, Jose M.
AU - Mousavi Khaneghah, Amin
AU - Tsatsanis, Christos
AU - Kampranis, Sotirios C.
AU - Barba, Francisco J.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - The recent progress in genetic engineering has brought multiple benefits to the food and agricultural industry by enhancing the essential characteristics of agronomic traits. Powerful tools in the field of genome editing, such as siRNA-mediated RNA interference for targeted suppression of gene expression and transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs) for DNA repair have been widely used for commercial purposes. However, in the last few years, the discovery of the CRISPR-Cas9 system has revolutionized genome editing and has attracted attention as a powerful tool for several industrial applications. Herein, we review current progresses in the utilization of the CRISPR-Cas9 system in the food and agricultural industry, particularly in the development of resistant crops with improved quality and productivity. We compare the CRISPR system with the TALEN and ZFN nucleases–based methods and highlight potential advantages and shortcomings. In addition, we explore the state of the global market and discuss the safety and ethical concerns associated with the application of this technology in the food and agricultural industry.
AB - The recent progress in genetic engineering has brought multiple benefits to the food and agricultural industry by enhancing the essential characteristics of agronomic traits. Powerful tools in the field of genome editing, such as siRNA-mediated RNA interference for targeted suppression of gene expression and transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs) for DNA repair have been widely used for commercial purposes. However, in the last few years, the discovery of the CRISPR-Cas9 system has revolutionized genome editing and has attracted attention as a powerful tool for several industrial applications. Herein, we review current progresses in the utilization of the CRISPR-Cas9 system in the food and agricultural industry, particularly in the development of resistant crops with improved quality and productivity. We compare the CRISPR system with the TALEN and ZFN nucleases–based methods and highlight potential advantages and shortcomings. In addition, we explore the state of the global market and discuss the safety and ethical concerns associated with the application of this technology in the food and agricultural industry.
KW - Agriculture
KW - Bioactive compounds
KW - CRISPR-Cas9
KW - Food applications
KW - Plant genome editing
KW - Single guide RNA
UR - http://www.scopus.com/inward/record.url?scp=85061829605&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2019.02.006
DO - 10.1016/j.biotechadv.2019.02.006
M3 - Review
C2 - 30779952
AN - SCOPUS:85061829605
SN - 0734-9750
VL - 37
SP - 410
EP - 421
JO - Biotechnology Advances
JF - Biotechnology Advances
IS - 3
ER -