The absorption of natural vitamin c in horses and anti-oxidative capacity: A randomised, controlled study on trotters during a three-month intervention period

TY - JOUR

T1 - The absorption of natural vitamin c in horses and anti-oxidative capacity

T2 - A randomised, controlled study on trotters during a three-month intervention period

AU - Winther, Kaj

AU - Kharazmi, Arsalan

AU - Hansen, Anne Sophie Vinther

AU - Falk-Rønne, J

PY - 2012

Y1 - 2012

N2 - The primary aim of the present study was to assess whether low dose vitamin C supplemented in its natural form, as a dried powder from selected subspecies of rosehip (Rosa canina), would result in a detectable increase in the concentration of blood vitamin C in horses supplemented daily for three months. In a secondary part of the study, the anti-oxidant capacity of the present treatment was also tested. Sixteen horses were randomly allocated to two groups and supplemented with either 25 g rosehip powder daily (equal to 125 mg natural vitamin C), or with 50 gram rosehip powder daily (equal to 250 mg vitamin C). Serum vitamin C was analysed both prior to supplementation and then again 14, 28 and 84 days following supplementation. Anti-oxidative capacity was assessed as the release of oxidative anions from polymorph-nucleated leucocytes prior to supplementation and after 14 and 84 days, respectively. Whilst there was a trend towards an increase in serum vitamin C concentration following 84 days of supplementation with 125 mg of natural vitamin C, this was not statistically significant. However, a significant increase in serum vitamin C concentration was observed following 14 days of supplementation with 250 mg vitamin C (P < 0.02). The magnitude of this increase was greater following 28 and 84 days of supplementation (P < 0.02 and P < 0.02), respectively. Additionally, serum vitamin C concentration was significantly higher following supplementation with 250 versus 125 mg of vitamin C daily (P < 0.03). The higher dose of natural vitamin C also resulted in a significant reduction in the release of oxidative anions (P < 0.001). These results suggest that natural vitamin C from rosehip is absorbed sufficiently to raise serum vitamin C concentration and to reduce the liberation of oxidative anions in horses supplemented with less than 500 mg daily.

AB - The primary aim of the present study was to assess whether low dose vitamin C supplemented in its natural form, as a dried powder from selected subspecies of rosehip (Rosa canina), would result in a detectable increase in the concentration of blood vitamin C in horses supplemented daily for three months. In a secondary part of the study, the anti-oxidant capacity of the present treatment was also tested. Sixteen horses were randomly allocated to two groups and supplemented with either 25 g rosehip powder daily (equal to 125 mg natural vitamin C), or with 50 gram rosehip powder daily (equal to 250 mg vitamin C). Serum vitamin C was analysed both prior to supplementation and then again 14, 28 and 84 days following supplementation. Anti-oxidative capacity was assessed as the release of oxidative anions from polymorph-nucleated leucocytes prior to supplementation and after 14 and 84 days, respectively. Whilst there was a trend towards an increase in serum vitamin C concentration following 84 days of supplementation with 125 mg of natural vitamin C, this was not statistically significant. However, a significant increase in serum vitamin C concentration was observed following 14 days of supplementation with 250 mg vitamin C (P < 0.02). The magnitude of this increase was greater following 28 and 84 days of supplementation (P < 0.02 and P < 0.02), respectively. Additionally, serum vitamin C concentration was significantly higher following supplementation with 250 versus 125 mg of vitamin C daily (P < 0.03). The higher dose of natural vitamin C also resulted in a significant reduction in the release of oxidative anions (P < 0.001). These results suggest that natural vitamin C from rosehip is absorbed sufficiently to raise serum vitamin C concentration and to reduce the liberation of oxidative anions in horses supplemented with less than 500 mg daily.

KW - Antioxidants

KW - Dietary supplements

KW - Horses

KW - Rosehip

KW - Vitamin C

U2 - 10.3920/CEP12006

DO - 10.3920/CEP12006

M3 - Journal article

AN - SCOPUS:84928403042

SN - 1755-2540

VL - 8

SP - 195

EP - 201

JO - Comparative Exercise Physiology

JF - Comparative Exercise Physiology

IS - 3-4

ER -