Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages

TY - JOUR

T1 - Th1-like human T-cell clones recognizing Leishmania gp63 inhibit Leishmania major in human macrophages

AU - Kemp, M

AU - Hey, A S

AU - Bendtzen, K

AU - Kharazmi, A

AU - Theander, T G

N1 - Keywords: Adult; Animals; Antigens, Protozoan; Clone Cells; Flow Cytometry; Humans; Interferon Type II; Interleukin-4; Leishmania major; Macrophages; Metalloendopeptidases; Th1 Cells

PY - 1994

Y1 - 1994

N2 - The major surface protease of Leishmania major, gp63, has been suggested as a vaccine candidate for cutaneous leishmaniasis. In this study gp63 was purified from L. major promastigotes. A panel of human T-cell clones recognizing this protein were generated from individuals who had previously had self-healing cutaneous leishmaniasis. The T-cell clones expressed CD4, and the alpha chain of the T-cell antigen receptor. GP63 reactive T-cell clones activated by antigen or by immobilized anti-CD3 antibody released relative large amounts of interferon-gamma and no or little interleukin-4, thereby resembling Th1 cells. Autologous mononuclear cells and Epstein-Barr virus-transformed B cell lines were equally efficient in presenting the antigen to the T cells. The gp63 reactive T cells induced resistance to infection in cultured human macrophages by L. major. The data confirm that human CD4+ T cells recognizing gp63 can take part in the host defence against L. major infections.

AB - The major surface protease of Leishmania major, gp63, has been suggested as a vaccine candidate for cutaneous leishmaniasis. In this study gp63 was purified from L. major promastigotes. A panel of human T-cell clones recognizing this protein were generated from individuals who had previously had self-healing cutaneous leishmaniasis. The T-cell clones expressed CD4, and the alpha chain of the T-cell antigen receptor. GP63 reactive T-cell clones activated by antigen or by immobilized anti-CD3 antibody released relative large amounts of interferon-gamma and no or little interleukin-4, thereby resembling Th1 cells. Autologous mononuclear cells and Epstein-Barr virus-transformed B cell lines were equally efficient in presenting the antigen to the T cells. The gp63 reactive T cells induced resistance to infection in cultured human macrophages by L. major. The data confirm that human CD4+ T cells recognizing gp63 can take part in the host defence against L. major infections.

M3 - Journal article

C2 - 7997852

SN - 0301-6323

VL - 40

SP - 629

EP - 635

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 6

ER -