TFAP2B influences the effect of dietary fat on weight loss under energy restriction

Tanja Stocks; Lars Angquist; Karina Banasik; Marie Neergaard Harder; Moira A Taylor; Jörg Hager; Peter Arner; Jean-Michel Oppert; J Alfredo Martinez; Jan Polak; Francis Rousseau; Dominique Langin; Stephan Rössner; Claus Holst; Ian A Macdonald; Yoichiro Kamatani; Andreas F H Pfeiffer; Marie Kunesova; Wim H M Saris; Torben Hansen; Oluf Pedersen; Arne Astrup; Thorkild I.A. Sørensen

doi:10.1371/journal.pone.0043212

TFAP2B influences the effect of dietary fat on weight loss under energy restriction

Tanja Stocks, Lars Angquist, Karina Banasik, Marie Neergaard Harder, Moira A Taylor, Jörg Hager, Peter Arner, Jean-Michel Oppert, J Alfredo Martinez, Jan Polak, Francis Rousseau, Dominique Langin, Stephan Rössner, Claus Holst, Ian A Macdonald, Yoichiro Kamatani, Andreas F H Pfeiffer, Marie Kunesova, Wim H M Saris, Torben HansenOluf Pedersen, Arne Astrup, Thorkild I.A. Sørensen

25 Citations (Scopus)

Abstract

Background: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. Methods and Findings: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. Conclusions: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.

Original language	English
Journal	P L o S One
Volume	7
Issue number	8
Pages (from-to)	e43212
Number of pages	10
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0043212
Publication status	Published - 27 Aug 2012

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Stocks, T., Angquist, L., Banasik, K., Harder, M. N., Taylor, M. A., Hager, J., Arner, P., Oppert, J-M., Martinez, J. A., Polak, J., Rousseau, F., Langin, D., Rössner, S., Holst, C., Macdonald, I. A., Kamatani, Y., Pfeiffer, A. F. H., Kunesova, M., Saris, W. H. M., ... Sørensen, T. I. A. (2012). TFAP2B influences the effect of dietary fat on weight loss under energy restriction. P L o S One, 7(8), e43212. https://doi.org/10.1371/journal.pone.0043212

Stocks, T, Angquist, L , Banasik, K, Harder, MN, Taylor, MA, Hager, J, Arner, P, Oppert, J-M, Martinez, JA, Polak, J, Rousseau, F, Langin, D, Rössner, S, Holst, C, Macdonald, IA, Kamatani, Y, Pfeiffer, AFH, Kunesova, M, Saris, WHM, Hansen, T , Pedersen, O, Astrup, A & Sørensen, TIA 2012, 'TFAP2B influences the effect of dietary fat on weight loss under energy restriction', P L o S One, vol. 7, no. 8, pp. e43212. https://doi.org/10.1371/journal.pone.0043212

@article{4f4c7ed8d8564feea06d6358e75edf2a,

title = "TFAP2B influences the effect of dietary fat on weight loss under energy restriction",

abstract = "Background: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. Methods and Findings: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. Conclusions: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.",

author = "Tanja Stocks and Lars Angquist and Karina Banasik and Harder, {Marie Neergaard} and Taylor, {Moira A} and J{\"o}rg Hager and Peter Arner and Jean-Michel Oppert and Martinez, {J Alfredo} and Jan Polak and Francis Rousseau and Dominique Langin and Stephan R{\"o}ssner and Claus Holst and Macdonald, {Ian A} and Yoichiro Kamatani and Pfeiffer, {Andreas F H} and Marie Kunesova and Saris, {Wim H M} and Torben Hansen and Oluf Pedersen and Arne Astrup and S{\o}rensen, {Thorkild I.A.}",

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TY - JOUR

T1 - TFAP2B influences the effect of dietary fat on weight loss under energy restriction

AU - Stocks, Tanja

AU - Angquist, Lars

AU - Banasik, Karina

AU - Harder, Marie Neergaard

AU - Taylor, Moira A

AU - Hager, Jörg

AU - Arner, Peter

AU - Oppert, Jean-Michel

AU - Martinez, J Alfredo

AU - Polak, Jan

AU - Rousseau, Francis

AU - Langin, Dominique

AU - Rössner, Stephan

AU - Holst, Claus

AU - Macdonald, Ian A

AU - Kamatani, Yoichiro

AU - Pfeiffer, Andreas F H

AU - Kunesova, Marie

AU - Saris, Wim H M

AU - Hansen, Torben

AU - Pedersen, Oluf

AU - Astrup, Arne

AU - Sørensen, Thorkild I.A.

N1 - IHE 2012 019

PY - 2012/8/27

Y1 - 2012/8/27

N2 - Background: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. Methods and Findings: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. Conclusions: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.

AB - Background: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. Methods and Findings: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. Conclusions: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.

U2 - 10.1371/journal.pone.0043212

DO - 10.1371/journal.pone.0043212

M3 - Journal article

C2 - 22952648

SN - 1932-6203

VL - 7

SP - e43212

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 8

ER -

TFAP2B influences the effect of dietary fat on weight loss under energy restriction

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