Tetranectin in slow intra- and extrafusal chicken muscle fibers.

TY - JOUR

T1 - Tetranectin in slow intra- and extrafusal chicken muscle fibers.

AU - Xu, X

AU - Gilpin, B

AU - Iba, K

AU - Maier, A

AU - Engvall, E

AU - Albrechtsen, R

AU - Wewer, U M

N1 - Keywords: Adaptation, Physiological; Amino Acid Sequence; Animals; Blood Proteins; Caenorhabditis elegans Proteins; Cattle; Cell Differentiation; Chick Embryo; Chickens; DNA, Complementary; Drosophila Proteins; Gene Expression Regulation, Developmental; Humans; Immunohistochemistry; Lectins; Lectins, C-Type; Mice; Molecular Sequence Data; Muscle Contraction; Muscle Fibers, Fast-Twitch; Muscle Fibers, Slow-Twitch; Muscle Spindles; Muscle, Skeletal; Myosin Heavy Chains; Phylogeny; RNA, Messenger; Sequence Homology, Nucleic Acid; Stem Cells

PY - 2001

Y1 - 2001

N2 - Tetranectin is a C-type lectin that occurs in the mammalian musculoskeletal system. In the present report we describe the first studies on an avian tetranectin. A full-length chicken tetranectin cDNA was isolated. Comparison of the deduced amino acid sequence of chicken tetranectin with mouse and human tetranectin showed an identity of 67 and 68%, respectively. Northern blot analysis demonstrated broad expression of chicken tetranectin mRNA, which was first detected on embryonic day 4. Tetranectin protein was detected in chicken serum and egg yolk. Since muscle is one of few tissues in which tetranectin protein is retained, we examined the distribution of tetranectin in various muscle types in chicken. Myofibers strongly positive for tetranectin were observed in several muscles including m. tibialis ant. and m. sartorius (from embryonic day 10 to adult). Using antibodies to fast and slow myosin heavy chains (MHC) and double immunostaining techniques, we found that tetranectin was restricted to slow (type I) muscle fibers. Similarly only slow intrafusal fibers accumulated tetranectin. The pattern of immunostaining in chickens differs markedly from that seen in mouse muscles, indicating that tetranectin performs a role in muscle that is not associated with a hitherto recognized muscle type or function.

AB - Tetranectin is a C-type lectin that occurs in the mammalian musculoskeletal system. In the present report we describe the first studies on an avian tetranectin. A full-length chicken tetranectin cDNA was isolated. Comparison of the deduced amino acid sequence of chicken tetranectin with mouse and human tetranectin showed an identity of 67 and 68%, respectively. Northern blot analysis demonstrated broad expression of chicken tetranectin mRNA, which was first detected on embryonic day 4. Tetranectin protein was detected in chicken serum and egg yolk. Since muscle is one of few tissues in which tetranectin protein is retained, we examined the distribution of tetranectin in various muscle types in chicken. Myofibers strongly positive for tetranectin were observed in several muscles including m. tibialis ant. and m. sartorius (from embryonic day 10 to adult). Using antibodies to fast and slow myosin heavy chains (MHC) and double immunostaining techniques, we found that tetranectin was restricted to slow (type I) muscle fibers. Similarly only slow intrafusal fibers accumulated tetranectin. The pattern of immunostaining in chickens differs markedly from that seen in mouse muscles, indicating that tetranectin performs a role in muscle that is not associated with a hitherto recognized muscle type or function.

M3 - Journal article

C2 - 11519735

SN - 0142-4319

VL - 22

SP - 121

EP - 132

JO - Journal of Muscle Research and Cell Motility

JF - Journal of Muscle Research and Cell Motility

IS - 2

ER -