Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus.

TS Hansen; PH Andreasen; H Dreisig; P Højrup; H Nielsen; J Engberg; Karsten Kristiansen

Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus.

TS Hansen, PH Andreasen, H Dreisig, P Højrup, H Nielsen, J Engberg, Karsten Kristiansen

18 Citations (Scopus)

Abstract

We have cloned and characterized a Tetrahymena thermophila macronuclear gene (L37) encoding the acidic ribosomal protein (A-protein) L37. The gene contains a single intron located in the 3'-part of the coding region. Two major and three minor transcription start points (tsp) were mapped 39 to 63 nucleotides upstream from the translational start codon. The uppermost tsp mapped to the first T in a putative T. thermophila RNA polymerase II initiator element, TATAA. The coding region of L37 predicts a protein of 109 amino acid (aa) residues. A substantial part of the deduced aa sequence was verified by protein sequencing. The T. thermophila L37 clearly belongs to the P1-type family of eukaryotic A-proteins, but the C-terminal region has the hallmarks of archaebacterial A-proteins.

Original language	English
Journal	Gene
Volume	105
Issue number	2
Pages (from-to)	143-50
ISSN	0378-1119
Publication status	Published - 1991
Externally published	Yes

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title = "Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus.",

abstract = "We have cloned and characterized a Tetrahymena thermophila macronuclear gene (L37) encoding the acidic ribosomal protein (A-protein) L37. The gene contains a single intron located in the 3'-part of the coding region. Two major and three minor transcription start points (tsp) were mapped 39 to 63 nucleotides upstream from the translational start codon. The uppermost tsp mapped to the first T in a putative T. thermophila RNA polymerase II initiator element, TATAA. The coding region of L37 predicts a protein of 109 amino acid (aa) residues. A substantial part of the deduced aa sequence was verified by protein sequencing. The T. thermophila L37 clearly belongs to the P1-type family of eukaryotic A-proteins, but the C-terminal region has the hallmarks of archaebacterial A-proteins.",

author = "TS Hansen and PH Andreasen and H Dreisig and P H{\o}jrup and H Nielsen and J Engberg and Karsten Kristiansen",

year = "1991",

language = "English",

volume = "105",

pages = "143--50",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus.

AU - Hansen, TS

AU - Andreasen, PH

AU - Dreisig, H

AU - Højrup, P

AU - Nielsen, H

AU - Engberg, J

AU - Kristiansen, Karsten

PY - 1991

Y1 - 1991

N2 - We have cloned and characterized a Tetrahymena thermophila macronuclear gene (L37) encoding the acidic ribosomal protein (A-protein) L37. The gene contains a single intron located in the 3'-part of the coding region. Two major and three minor transcription start points (tsp) were mapped 39 to 63 nucleotides upstream from the translational start codon. The uppermost tsp mapped to the first T in a putative T. thermophila RNA polymerase II initiator element, TATAA. The coding region of L37 predicts a protein of 109 amino acid (aa) residues. A substantial part of the deduced aa sequence was verified by protein sequencing. The T. thermophila L37 clearly belongs to the P1-type family of eukaryotic A-proteins, but the C-terminal region has the hallmarks of archaebacterial A-proteins.

AB - We have cloned and characterized a Tetrahymena thermophila macronuclear gene (L37) encoding the acidic ribosomal protein (A-protein) L37. The gene contains a single intron located in the 3'-part of the coding region. Two major and three minor transcription start points (tsp) were mapped 39 to 63 nucleotides upstream from the translational start codon. The uppermost tsp mapped to the first T in a putative T. thermophila RNA polymerase II initiator element, TATAA. The coding region of L37 predicts a protein of 109 amino acid (aa) residues. A substantial part of the deduced aa sequence was verified by protein sequencing. The T. thermophila L37 clearly belongs to the P1-type family of eukaryotic A-proteins, but the C-terminal region has the hallmarks of archaebacterial A-proteins.

M3 - Journal article

SN - 0378-1119

VL - 105

SP - 143

EP - 150

JO - Gene

JF - Gene

IS - 2

ER -

Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus.

Abstract

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