TY - JOUR
T1 - Temperature dependent embryonic development of Trichuris suis eggs in a medicinal raw material
AU - Vejzagic, Nermina
AU - Kringel, Helene
AU - Bruun, Johan Musaeus
AU - Roepstorff, Allan
AU - Thamsborg, Stig Milan
AU - Grossi, Anette Blak
AU - Kapel, Christian Moliin Outzen
PY - 2016
Y1 - 2016
N2 - The therapeutic potential of infective pig whipworm eggs, Trichuris suis ova (TSO), is currently tested in several clinical trials on immune-mediated diseases. This paper studied the embryonic development of TSO in a medicinal raw product, where the parasite eggs were suspended in sulphuric acid (pH1). Unembryonated T. suis egg batches were stored at 5, 10, 15, 20, 25, 30, and 40 °C (±1 °C) and examined at 2, 4, 8, and 14 weeks. Subsequently, sub-batches from each temperature were allowed to embryonate for additional 14 weeks at 25 °C, and selected samples were tested for infectivity in Göttingen minipigs.Both male and female pigs were used to evaluate eventual gender specific infectivity. Storage at 30 °C up to 14 weeks and subsequent embryonation for 14 weeks at 25 °C did not significantly reduce the overall larval establishment in minipigs, as compared to storage at 5 °C and subsequent embryonation at 25 °C. As marked impairment of egg development was observed during storage at 40 °C, a second set of unembryonated egg batches were incubated at 30, 32, 34, 36, 38, and 40 °C (±1 °C) for 1-8 weeks. The development of the eggs was repeatedly examined by manual light microscopy, multispectral analysis (OvaSpec), and an egg hatching assay prior to the final testing in minipigs (Trial 1). These methods showed that the development started earlier at higher temperatures, but the long-term storage at higher temperature affected the egg development. The present study further documents tolerance of the TSO to storage at temperature 5-15 °C, at which temperature development of larvae is not initiated.
AB - The therapeutic potential of infective pig whipworm eggs, Trichuris suis ova (TSO), is currently tested in several clinical trials on immune-mediated diseases. This paper studied the embryonic development of TSO in a medicinal raw product, where the parasite eggs were suspended in sulphuric acid (pH1). Unembryonated T. suis egg batches were stored at 5, 10, 15, 20, 25, 30, and 40 °C (±1 °C) and examined at 2, 4, 8, and 14 weeks. Subsequently, sub-batches from each temperature were allowed to embryonate for additional 14 weeks at 25 °C, and selected samples were tested for infectivity in Göttingen minipigs.Both male and female pigs were used to evaluate eventual gender specific infectivity. Storage at 30 °C up to 14 weeks and subsequent embryonation for 14 weeks at 25 °C did not significantly reduce the overall larval establishment in minipigs, as compared to storage at 5 °C and subsequent embryonation at 25 °C. As marked impairment of egg development was observed during storage at 40 °C, a second set of unembryonated egg batches were incubated at 30, 32, 34, 36, 38, and 40 °C (±1 °C) for 1-8 weeks. The development of the eggs was repeatedly examined by manual light microscopy, multispectral analysis (OvaSpec), and an egg hatching assay prior to the final testing in minipigs (Trial 1). These methods showed that the development started earlier at higher temperatures, but the long-term storage at higher temperature affected the egg development. The present study further documents tolerance of the TSO to storage at temperature 5-15 °C, at which temperature development of larvae is not initiated.
KW - Egg development
KW - Embryonation
KW - Göttingen minipigs
KW - Stability-indicating assays
KW - Temperature
KW - Trichuris suis ova
U2 - 10.1016/j.vetpar.2015.10.031
DO - 10.1016/j.vetpar.2015.10.031
M3 - Journal article
C2 - 26790737
AN - SCOPUS:84958781122
SN - 0304-4017
VL - 215
SP - 48
EP - 57
JO - Veterinary Parasitology
JF - Veterinary Parasitology
ER -