T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression

H Aladdin; T Katzenstein; A.-M. Dreves; L Ryder; J Gerstoft; P Skinhøj; Bente Klarlund Pedersen; H Ullum

T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression

H Aladdin, T Katzenstein, A.-M. Dreves, L Ryder, J Gerstoft, P Skinhøj, Bente Klarlund Pedersen, H Ullum

Department of Clinical Medicine

10 Citations (Scopus)

Abstract

This study evaluated the effect of highly active antiretroviral therapy (HAART)-induced viral suppression on T-cell receptor excisional circles (TRECs), telomere length, proliferative responses and spontaneous as well as phytohaemagglutinin (PHA)-stimulated lymphocyte apoptosis in 27 human immunodeficiency virus (HIV)-infected individuals followed for 18 months during HAART. Our results show that HAART significantly increased the level of TRECs in CD4+ cells (P = 0.003) after 18 months of almost continuously suppressed HIV-RNA levels. Lymphocyte proliferative responses and apoptosis levels in patients were significantly lower and significantly higher, respectively, compared with healthy controls. The proliferative response and apoptosis levels did not change during follow up. Changes in telomere length were observed in CD4+ and in CD8+ T cells. The study demonstrated that HAART induces normal TREC levels in the CD4+ T-cell pool. However, the other perturbed functions in T cells indicate that immune reconstitution is incomplete and may need longer viral suppression.

Original language	English
Journal	Scandinavian Journal of Immunology
Volume	57
Issue number	5
Pages (from-to)	485-92
Number of pages	8
ISSN	0300-9475
Publication status	Published - May 2003

Keywords

Adult
Aged
Anti-HIV Agents
Antiretroviral Therapy, Highly Active
Apoptosis
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
DNA
Female
Follow-Up Studies
Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
HIV Infections
Humans
Lymphocyte Activation
Male
Middle Aged
Plasmids
Receptors, Antigen, T-Cell, alpha-beta
Telomere
Viral Load
Journal Article

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Aladdin, H., Katzenstein, T., Dreves, A-M., Ryder, L., Gerstoft, J., Skinhøj, P., Pedersen, B. K., & Ullum, H. (2003). T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression. Scandinavian Journal of Immunology, 57(5), 485-92.

T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression. / Aladdin, H; Katzenstein, T; Dreves, A.-M. et al.

In: Scandinavian Journal of Immunology, Vol. 57, No. 5, 05.2003, p. 485-92.

Research output: Contribution to journal › Journal article › Research › peer-review

Aladdin, H, Katzenstein, T, Dreves, A-M, Ryder, L, Gerstoft, J, Skinhøj, P, Pedersen, BK & Ullum, H 2003, 'T-cell receptor excisional circles, telomere length, proliferation and apoptosis in peripheral blood mononuclear cells of human immunodeficiency virus-infected individuals after 18 months of treatment induced viral suppression', Scandinavian Journal of Immunology, vol. 57, no. 5, pp. 485-92.

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abstract = "This study evaluated the effect of highly active antiretroviral therapy (HAART)-induced viral suppression on T-cell receptor excisional circles (TRECs), telomere length, proliferative responses and spontaneous as well as phytohaemagglutinin (PHA)-stimulated lymphocyte apoptosis in 27 human immunodeficiency virus (HIV)-infected individuals followed for 18 months during HAART. Our results show that HAART significantly increased the level of TRECs in CD4+ cells (P = 0.003) after 18 months of almost continuously suppressed HIV-RNA levels. Lymphocyte proliferative responses and apoptosis levels in patients were significantly lower and significantly higher, respectively, compared with healthy controls. The proliferative response and apoptosis levels did not change during follow up. Changes in telomere length were observed in CD4+ and in CD8+ T cells. The study demonstrated that HAART induces normal TREC levels in the CD4+ T-cell pool. However, the other perturbed functions in T cells indicate that immune reconstitution is incomplete and may need longer viral suppression.",

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AU - Dreves, A.-M.

AU - Ryder, L

AU - Gerstoft, J

AU - Skinhøj, P

AU - Pedersen, Bente Klarlund

AU - Ullum, H

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AB - This study evaluated the effect of highly active antiretroviral therapy (HAART)-induced viral suppression on T-cell receptor excisional circles (TRECs), telomere length, proliferative responses and spontaneous as well as phytohaemagglutinin (PHA)-stimulated lymphocyte apoptosis in 27 human immunodeficiency virus (HIV)-infected individuals followed for 18 months during HAART. Our results show that HAART significantly increased the level of TRECs in CD4+ cells (P = 0.003) after 18 months of almost continuously suppressed HIV-RNA levels. Lymphocyte proliferative responses and apoptosis levels in patients were significantly lower and significantly higher, respectively, compared with healthy controls. The proliferative response and apoptosis levels did not change during follow up. Changes in telomere length were observed in CD4+ and in CD8+ T cells. The study demonstrated that HAART induces normal TREC levels in the CD4+ T-cell pool. However, the other perturbed functions in T cells indicate that immune reconstitution is incomplete and may need longer viral suppression.

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