Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation

Shankha Satpathy; Sebastian A Wagner; Petra Beli; Rajat Gupta; Trine A Kristiansen; Dessislava Malinova; Chiara Francavilla; Pavel Tolar; Gail A Bishop; Bruce S Hostager; Chuna Ram Choudhary

doi:10.15252/msb.20145880

Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation

Shankha Satpathy, Sebastian A Wagner, Petra Beli, Rajat Gupta, Trine A Kristiansen, Dessislava Malinova, Chiara Francavilla, Pavel Tolar, Gail A Bishop, Bruce S Hostager, Chuna Ram Choudhary

65 Citations (Scopus)

Abstract

B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR-induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo-lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC-mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR-induced NF-κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks. Synopsis Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system. Quantitative analysis of BCR signaling reveals the dynamics of BCR signalosome, ubiquitylome, and phosphoproteome. BCR-induced phosphorylation of RAB7A controls its binding to endosomes. BCR stimulation increases linear ubiquitylation of BCL10 and activation of NF-κB signaling. HOIP and TRAF6 are involved in BCR-induced ubiquitylation of BCL10. Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system.

Original language	English
Article number	810
Journal	Molecular Systems Biology
Volume	11
Issue number	6
ISSN	1744-4292
DOIs	https://doi.org/10.15252/msb.20145880
Publication status	Published - 1 Jun 2015

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@article{764efab2879c4228ac54497b0aa67cac,

title = "Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation",

abstract = "B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR-induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo-lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC-mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR-induced NF-κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks. Synopsis Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system. Quantitative analysis of BCR signaling reveals the dynamics of BCR signalosome, ubiquitylome, and phosphoproteome. BCR-induced phosphorylation of RAB7A controls its binding to endosomes. BCR stimulation increases linear ubiquitylation of BCL10 and activation of NF-κB signaling. HOIP and TRAF6 are involved in BCR-induced ubiquitylation of BCL10. Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system.",

author = "Shankha Satpathy and Wagner, {Sebastian A} and Petra Beli and Rajat Gupta and Kristiansen, {Trine A} and Dessislava Malinova and Chiara Francavilla and Pavel Tolar and Bishop, {Gail A} and Hostager, {Bruce S} and Choudhary, {Chuna Ram}",

note = "{\textcopyright} 2015 The Authors. Published under the terms of the CC BY 4.0 license.",

year = "2015",

month = jun,

day = "1",

doi = "10.15252/msb.20145880",

language = "English",

volume = "11",

journal = "Molecular Systems Biology",

issn = "1744-4292",

publisher = "Wiley-Blackwell",

number = "6",

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TY - JOUR

T1 - Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation

AU - Satpathy, Shankha

AU - Wagner, Sebastian A

AU - Beli, Petra

AU - Gupta, Rajat

AU - Kristiansen, Trine A

AU - Malinova, Dessislava

AU - Francavilla, Chiara

AU - Tolar, Pavel

AU - Bishop, Gail A

AU - Hostager, Bruce S

AU - Choudhary, Chuna Ram

PY - 2015/6/1

Y1 - 2015/6/1

N2 - B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR-induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo-lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC-mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR-induced NF-κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks. Synopsis Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system. Quantitative analysis of BCR signaling reveals the dynamics of BCR signalosome, ubiquitylome, and phosphoproteome. BCR-induced phosphorylation of RAB7A controls its binding to endosomes. BCR stimulation increases linear ubiquitylation of BCL10 and activation of NF-κB signaling. HOIP and TRAF6 are involved in BCR-induced ubiquitylation of BCL10. Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system.

AB - B-cell receptor (BCR) signaling is essential for the development and function of B cells; however, the spectrum of proteins involved in BCR signaling is not fully known. Here we used quantitative mass spectrometry-based proteomics to monitor the dynamics of BCR signaling complexes (signalosomes) and to investigate the dynamics of downstream phosphorylation and ubiquitylation signaling. We identify most of the previously known components of BCR signaling, as well as many proteins that have not yet been implicated in this system. BCR activation leads to rapid tyrosine phosphorylation and ubiquitylation of the receptor-proximal signaling components, many of which are co-regulated by both the modifications. We illustrate the power of multilayered proteomic analyses for discovering novel BCR signaling components by demonstrating that BCR-induced phosphorylation of RAB7A at S72 prevents its association with effector proteins and with endo-lysosomal compartments. In addition, we show that BCL10 is modified by LUBAC-mediated linear ubiquitylation, and demonstrate an important function of LUBAC in BCR-induced NF-κB signaling. Our results offer a global and integrated view of BCR signaling, and the provided datasets can serve as a valuable resource for further understanding BCR signaling networks. Synopsis Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system. Quantitative analysis of BCR signaling reveals the dynamics of BCR signalosome, ubiquitylome, and phosphoproteome. BCR-induced phosphorylation of RAB7A controls its binding to endosomes. BCR stimulation increases linear ubiquitylation of BCL10 and activation of NF-κB signaling. HOIP and TRAF6 are involved in BCR-induced ubiquitylation of BCL10. Mass spectrometry-based quantitative analysis of B-cell receptor (BCR) signaling provides a global view of BCR signaling and identifies novel regulatory functions of phosphorylation and ubiquitylation in this system.

U2 - 10.15252/msb.20145880

DO - 10.15252/msb.20145880

M3 - Journal article

C2 - 26038114

SN - 1744-4292

VL - 11

JO - Molecular Systems Biology

JF - Molecular Systems Biology

IS - 6

M1 - 810

ER -

Systems-wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation

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