TY - JOUR
T1 - Systematic review of survival time in experimental mouse stroke with impact on reliability of infarct estimation
AU - Klarskov, Carina Kirstine
AU - Klarskov, Mikkel Buster
AU - Hasseldam, Henrik
AU - Johansen, Flemming Fryd
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Background: Stroke is the second most common cause of death worldwide. Only one treatment for acute ischemic stroke is currently available, thrombolysis with rt-PA, but it is limited in its use. Many efforts have been invested in order to find additive treatments, without success.A multitude of reasons for the translational problems from mouse experimental stroke to clinical trials probably exists, including infarct size estimations around the peak time of edema formation. Furthermore, edema is a more prominent feature of stroke in mice than in humans, because of the tendency to produce larger infarcts with more substantial edema. Purpose: This paper will give an overview of previous studies of experimental mouse stroke, and correlate survival time to peak time of edema formation. Furthermore, investigations of whether the included studies corrected the infarct measurements for edema and a comparison of correction methods will be discussed. Method: Relevant terms were searched in the National Library of Medicine PubMed database. A method for classification of infarct measurement methods was made using a naming convention. Conclusion: Our study shows that infarct size estimations are often performed around the peak time of edema, with a median of 24. h. Most studies do consider edema formation, however, there is no consensus on what method to use to correct for edema. Furthermore, investigations into neuroprotective drugs should use longer survival times to ensure completion of the investigated process. Our findings indicate a need for more research in this area, and establishment of common correction methodology.
AB - Background: Stroke is the second most common cause of death worldwide. Only one treatment for acute ischemic stroke is currently available, thrombolysis with rt-PA, but it is limited in its use. Many efforts have been invested in order to find additive treatments, without success.A multitude of reasons for the translational problems from mouse experimental stroke to clinical trials probably exists, including infarct size estimations around the peak time of edema formation. Furthermore, edema is a more prominent feature of stroke in mice than in humans, because of the tendency to produce larger infarcts with more substantial edema. Purpose: This paper will give an overview of previous studies of experimental mouse stroke, and correlate survival time to peak time of edema formation. Furthermore, investigations of whether the included studies corrected the infarct measurements for edema and a comparison of correction methods will be discussed. Method: Relevant terms were searched in the National Library of Medicine PubMed database. A method for classification of infarct measurement methods was made using a naming convention. Conclusion: Our study shows that infarct size estimations are often performed around the peak time of edema, with a median of 24. h. Most studies do consider edema formation, however, there is no consensus on what method to use to correct for edema. Furthermore, investigations into neuroprotective drugs should use longer survival times to ensure completion of the investigated process. Our findings indicate a need for more research in this area, and establishment of common correction methodology.
KW - Brain
KW - Edema
KW - Infarct
KW - Ischemia
KW - Mouse
KW - Stroke
U2 - 10.1016/j.jneumeth.2015.11.010
DO - 10.1016/j.jneumeth.2015.11.010
M3 - Review
C2 - 26620203
AN - SCOPUS:84953725363
SN - 0165-0270
VL - 261
SP - 10
EP - 18
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
ER -