Synthetic Protein Scaffolding at Biological Membranes

James Bruce Yarnton H Behrendorff; Guillem Borràs I Gas; Mathias Pribil

doi:10.1016/j.tibtech.2019.10.009

Synthetic Protein Scaffolding at Biological Membranes

James Bruce Yarnton H Behrendorff, Guillem Borràs I Gas, Mathias Pribil^*

^*Corresponding author for this work

Section for Molecular Plant Biology

9 Citations (Scopus)

Abstract

Protein scaffolding is a natural phenomenon whereby proteins colocalize into macromolecular complexes via specific protein–protein interactions. In the case of metabolic enzymes, protein scaffolding drives metabolic flux through specific pathways by colocalizing enzyme active sites. Synthetic protein scaffolding is increasingly used as a mechanism to improve product specificity and yields in metabolic engineering projects. To date, synthetic scaffolding has focused primarily on soluble enzyme systems, but many metabolic pathways for high-value secondary metabolites depend on membrane-bound enzymes. The compositional diversity of biological membranes and general challenges associated with modifying membrane proteins complicate scaffolding with membrane-requiring enzymes. Several recent studies have introduced new approaches to protein scaffolding at membrane surfaces, with notable success in improving product yields from specific metabolic pathways.

Original language	English
Journal	Trends in Biotechnology
ISSN	0167-7799
DOIs	https://doi.org/10.1016/j.tibtech.2019.10.009
Publication status	Published - Apr 2020

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title = "Synthetic Protein Scaffolding at Biological Membranes",

abstract = "Protein scaffolding is a natural phenomenon whereby proteins colocalize into macromolecular complexes via specific protein–protein interactions. In the case of metabolic enzymes, protein scaffolding drives metabolic flux through specific pathways by colocalizing enzyme active sites. Synthetic protein scaffolding is increasingly used as a mechanism to improve product specificity and yields in metabolic engineering projects. To date, synthetic scaffolding has focused primarily on soluble enzyme systems, but many metabolic pathways for high-value secondary metabolites depend on membrane-bound enzymes. The compositional diversity of biological membranes and general challenges associated with modifying membrane proteins complicate scaffolding with membrane-requiring enzymes. Several recent studies have introduced new approaches to protein scaffolding at membrane surfaces, with notable success in improving product yields from specific metabolic pathways.",

author = "Behrendorff, {James Bruce Yarnton H} and {Borr{\`a}s I Gas}, Guillem and Mathias Pribil",

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language = "English",

journal = "Trends in Biotechnology",

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T1 - Synthetic Protein Scaffolding at Biological Membranes

AU - Behrendorff, James Bruce Yarnton H

AU - Borràs I Gas, Guillem

AU - Pribil, Mathias

PY - 2020/4

Y1 - 2020/4

N2 - Protein scaffolding is a natural phenomenon whereby proteins colocalize into macromolecular complexes via specific protein–protein interactions. In the case of metabolic enzymes, protein scaffolding drives metabolic flux through specific pathways by colocalizing enzyme active sites. Synthetic protein scaffolding is increasingly used as a mechanism to improve product specificity and yields in metabolic engineering projects. To date, synthetic scaffolding has focused primarily on soluble enzyme systems, but many metabolic pathways for high-value secondary metabolites depend on membrane-bound enzymes. The compositional diversity of biological membranes and general challenges associated with modifying membrane proteins complicate scaffolding with membrane-requiring enzymes. Several recent studies have introduced new approaches to protein scaffolding at membrane surfaces, with notable success in improving product yields from specific metabolic pathways.

AB - Protein scaffolding is a natural phenomenon whereby proteins colocalize into macromolecular complexes via specific protein–protein interactions. In the case of metabolic enzymes, protein scaffolding drives metabolic flux through specific pathways by colocalizing enzyme active sites. Synthetic protein scaffolding is increasingly used as a mechanism to improve product specificity and yields in metabolic engineering projects. To date, synthetic scaffolding has focused primarily on soluble enzyme systems, but many metabolic pathways for high-value secondary metabolites depend on membrane-bound enzymes. The compositional diversity of biological membranes and general challenges associated with modifying membrane proteins complicate scaffolding with membrane-requiring enzymes. Several recent studies have introduced new approaches to protein scaffolding at membrane surfaces, with notable success in improving product yields from specific metabolic pathways.

U2 - 10.1016/j.tibtech.2019.10.009

DO - 10.1016/j.tibtech.2019.10.009

M3 - Review

C2 - 31718802

SN - 0167-7799

JO - Trends in Biotechnology

JF - Trends in Biotechnology

ER -

Synthetic Protein Scaffolding at Biological Membranes

Abstract

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