Synthesis of 1,2-phenylenediamine capturing molecule for the detection of diacetyl

TY - JOUR

T1 - Synthesis of 1,2-phenylenediamine capturing molecule for the detection of diacetyl

AU - Marri, Lucia

AU - Jansson, Anita M.

AU - Christensen, Caspar Elo

AU - Hindsgaul, Ole

PY - 2017/12

Y1 - 2017/12

N2 - Here we describe the design of 1,2-phenylenediamine capturing molecule and the synthesis steps necessary for its preparation. The designed 1,2-phenylenediamine derivative is able to capture diacetyl in solution, as shown by ESIMS, forming a chemical adduct, 1-4-quinoxaline. The methyl esters of diacetyl-adduct (DAA) and pentanedione-adduct (PDA) are incorporated to the lysines in BSA and the conjugate used for antibody screening and selection. In the research article is described an enzyme-linked immunosorbent assay developed to detect and quantify diacetyl in complex media.

AB - Here we describe the design of 1,2-phenylenediamine capturing molecule and the synthesis steps necessary for its preparation. The designed 1,2-phenylenediamine derivative is able to capture diacetyl in solution, as shown by ESIMS, forming a chemical adduct, 1-4-quinoxaline. The methyl esters of diacetyl-adduct (DAA) and pentanedione-adduct (PDA) are incorporated to the lysines in BSA and the conjugate used for antibody screening and selection. In the research article is described an enzyme-linked immunosorbent assay developed to detect and quantify diacetyl in complex media.

U2 - 10.1016/j.dib.2017.09.072

DO - 10.1016/j.dib.2017.09.072

M3 - Journal article

C2 - 29062874

SN - 2352-3409

VL - 15

SP - 483

EP - 490

JO - Data in Brief

JF - Data in Brief

ER -