Synchrotron radiation μCT and histology evaluation of bone-to-implant contact

Camilla Albeck Neldam; Jon Sporring; Alexander Rack; Torsten Lauridsen; Ellen Margrethe Hauge; Henrik L. Jørgensen; Niklas Rye Jørgensen; Robert Krarup Feidenhans'l; Else Marie Pinholt

doi:10.1016/j.jcms.2017.05.019

Synchrotron radiation μCT and histology evaluation of bone-to-implant contact

Camilla Albeck Neldam, Jon Sporring, Alexander Rack, Torsten Lauridsen, Ellen Margrethe Hauge, Henrik L. Jørgensen, Niklas Rye Jørgensen, Robert Krarup Feidenhans'l, Else Marie Pinholt

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Abstract

The purpose of this study was to evaluate bone-to-implant contact (BIC) in two-dimensional (2D) histology compared to high-resolution three-dimensional (3D) synchrotron radiation micro computed tomography (SR micro-CT). High spatial resolution, excellent signal-to-noise ratio, and contrast establish SR micro-CT as the leading imaging modality for hard X-ray microtomography. Using SR micro-CT at voxel size 5 μm in an experimental goat mandible model, no statistically significant difference was found between the different treatment modalities nor between recipient and reconstructed bone. The histological evaluation showed a statistically significant difference between BIC in reconstructed and recipient bone (p < 0.0001). Further, no statistically significant difference was found between the different treatment modalities which we found was due to large variation and subsequently due to low power. Comparing histology and SR micro-CT evaluation a bias of 5.2% was found in reconstructed area, and 15.3% in recipient bone. We conclude that for evaluation of BIC with histology and SR micro-CT, SR micro-CT cannot be proven more precise than histology for evaluation of BIC, however, with this SR micro-CT method, one histologic bone section is comparable to the 3D evaluation. Further, the two methods complement each other with knowledge on BIC in 2D and 3D.

Original language	English
Journal	Journal of Cranio-Maxillofacial Surgery
Volume	45
Issue number	9
Pages (from-to)	1448-1457
Number of pages	10
ISSN	1010-5182
DOIs	https://doi.org/10.1016/j.jcms.2017.05.019
Publication status	Published - Sept 2017

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@article{800eb30fa4654906bd6f2c2513c1fcd7,

title = "Synchrotron radiation μCT and histology evaluation of bone-to-implant contact",

abstract = "The purpose of this study was to evaluate bone-to-implant contact (BIC) in two-dimensional (2D) histology compared to high-resolution three-dimensional (3D) synchrotron radiation micro computed tomography (SR micro-CT). High spatial resolution, excellent signal-to-noise ratio, and contrast establish SR micro-CT as the leading imaging modality for hard X-ray microtomography. Using SR micro-CT at voxel size 5 μm in an experimental goat mandible model, no statistically significant difference was found between the different treatment modalities nor between recipient and reconstructed bone. The histological evaluation showed a statistically significant difference between BIC in reconstructed and recipient bone (p < 0.0001). Further, no statistically significant difference was found between the different treatment modalities which we found was due to large variation and subsequently due to low power. Comparing histology and SR micro-CT evaluation a bias of 5.2% was found in reconstructed area, and 15.3% in recipient bone. We conclude that for evaluation of BIC with histology and SR micro-CT, SR micro-CT cannot be proven more precise than histology for evaluation of BIC, however, with this SR micro-CT method, one histologic bone section is comparable to the 3D evaluation. Further, the two methods complement each other with knowledge on BIC in 2D and 3D.",

author = "Neldam, {Camilla Albeck} and Jon Sporring and Alexander Rack and Torsten Lauridsen and Hauge, {Ellen Margrethe} and J{\o}rgensen, {Henrik L.} and J{\o}rgensen, {Niklas Rye} and Feidenhans'l, {Robert Krarup} and Pinholt, {Else Marie}",

year = "2017",

month = sep,

doi = "10.1016/j.jcms.2017.05.019",

language = "English",

volume = "45",

pages = "1448--1457",

journal = "Journal of Cranio-Maxillofacial Surgery",

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T1 - Synchrotron radiation μCT and histology evaluation of bone-to-implant contact

AU - Neldam, Camilla Albeck

AU - Sporring, Jon

AU - Rack, Alexander

AU - Lauridsen, Torsten

AU - Hauge, Ellen Margrethe

AU - Jørgensen, Henrik L.

AU - Jørgensen, Niklas Rye

AU - Feidenhans'l, Robert Krarup

AU - Pinholt, Else Marie

PY - 2017/9

Y1 - 2017/9

N2 - The purpose of this study was to evaluate bone-to-implant contact (BIC) in two-dimensional (2D) histology compared to high-resolution three-dimensional (3D) synchrotron radiation micro computed tomography (SR micro-CT). High spatial resolution, excellent signal-to-noise ratio, and contrast establish SR micro-CT as the leading imaging modality for hard X-ray microtomography. Using SR micro-CT at voxel size 5 μm in an experimental goat mandible model, no statistically significant difference was found between the different treatment modalities nor between recipient and reconstructed bone. The histological evaluation showed a statistically significant difference between BIC in reconstructed and recipient bone (p < 0.0001). Further, no statistically significant difference was found between the different treatment modalities which we found was due to large variation and subsequently due to low power. Comparing histology and SR micro-CT evaluation a bias of 5.2% was found in reconstructed area, and 15.3% in recipient bone. We conclude that for evaluation of BIC with histology and SR micro-CT, SR micro-CT cannot be proven more precise than histology for evaluation of BIC, however, with this SR micro-CT method, one histologic bone section is comparable to the 3D evaluation. Further, the two methods complement each other with knowledge on BIC in 2D and 3D.

AB - The purpose of this study was to evaluate bone-to-implant contact (BIC) in two-dimensional (2D) histology compared to high-resolution three-dimensional (3D) synchrotron radiation micro computed tomography (SR micro-CT). High spatial resolution, excellent signal-to-noise ratio, and contrast establish SR micro-CT as the leading imaging modality for hard X-ray microtomography. Using SR micro-CT at voxel size 5 μm in an experimental goat mandible model, no statistically significant difference was found between the different treatment modalities nor between recipient and reconstructed bone. The histological evaluation showed a statistically significant difference between BIC in reconstructed and recipient bone (p < 0.0001). Further, no statistically significant difference was found between the different treatment modalities which we found was due to large variation and subsequently due to low power. Comparing histology and SR micro-CT evaluation a bias of 5.2% was found in reconstructed area, and 15.3% in recipient bone. We conclude that for evaluation of BIC with histology and SR micro-CT, SR micro-CT cannot be proven more precise than histology for evaluation of BIC, however, with this SR micro-CT method, one histologic bone section is comparable to the 3D evaluation. Further, the two methods complement each other with knowledge on BIC in 2D and 3D.

U2 - 10.1016/j.jcms.2017.05.019

DO - 10.1016/j.jcms.2017.05.019

M3 - Journal article

C2 - 28693947

SN - 1010-5182

VL - 45

SP - 1448

EP - 1457

JO - Journal of Cranio-Maxillofacial Surgery

JF - Journal of Cranio-Maxillofacial Surgery

IS - 9

ER -

Synchrotron radiation μCT and histology evaluation of bone-to-implant contact

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