Surface motility in Pseudomonas sp. DSS73 is required for efficient biological containment of the root-pathogenic microfungi Rhizoctonia solani and Pythium ultimum

TY - JOUR

T1 - Surface motility in Pseudomonas sp. DSS73 is required for efficient biological containment of the root-pathogenic microfungi Rhizoctonia solani and Pythium ultimum

AU - Andersen, Jens Bo

AU - Koch, Birgit

AU - Nielsen, Tommy Harder

AU - Sørensen, Dan

AU - Hansen, Michael

AU - Nybroe, Ole

AU - Christophersen, Carsten

AU - Sørensen, Jan

AU - Molin, Søren

AU - Givskov, Michael

N1 - Keywords: Antibiosis; Bacterial Proteins; Beta vulgaris; Culture Media; Movement; Peptides, Cyclic; Pest Control, Biological; Plant Diseases; Plant Roots; Pseudomonas; Pythium; Rhizoctonia; Soil Microbiology; Transcription Factors

PY - 2003

Y1 - 2003

N2 - Pseudomonas sp. DSS73 was isolated from the rhizoplane of sugar beet seedlings. This strain exhibits antagonism towards the root-pathogenic microfungi Pythium ultimum and Rhizoctonia solani. Production of the cyclic lipopeptide amphisin in combination with expression of flagella enables the growing bacterial culture to move readily over the surface of laboratory media. Amphisin is a new member of a group of dual-functioning compounds such as tensin, viscosin and viscosinamid that display both biosurfactant and antifungal properties. The ability of DSS73 to efficiently contain root-pathogenic microfungi is shown to arise from amphisin-dependent surface translocation and growth by which the bacterium can lay siege to the fungi. The synergistic effects of surface motility and synthesis of a battery of antifungal compounds efficiently contain and terminate growth of the microfungi.

AB - Pseudomonas sp. DSS73 was isolated from the rhizoplane of sugar beet seedlings. This strain exhibits antagonism towards the root-pathogenic microfungi Pythium ultimum and Rhizoctonia solani. Production of the cyclic lipopeptide amphisin in combination with expression of flagella enables the growing bacterial culture to move readily over the surface of laboratory media. Amphisin is a new member of a group of dual-functioning compounds such as tensin, viscosin and viscosinamid that display both biosurfactant and antifungal properties. The ability of DSS73 to efficiently contain root-pathogenic microfungi is shown to arise from amphisin-dependent surface translocation and growth by which the bacterium can lay siege to the fungi. The synergistic effects of surface motility and synthesis of a battery of antifungal compounds efficiently contain and terminate growth of the microfungi.

U2 - 10.1099/mic.0.25859-0

DO - 10.1099/mic.0.25859-0

M3 - Journal article

C2 - 12576578

SN - 1350-0872

VL - 149

SP - 37

EP - 46

JO - Microbiology

JF - Microbiology

IS - Pt 1

ER -