Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

Clare Louise Hawkins; Martin D Rees; Michael Jonathan Davies

Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

Clare Louise Hawkins, Martin D Rees, Michael Jonathan Davies

Inflammation, Metabolism and Oxidation

39 Citations (Scopus)

Abstract

Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.

Original language	English
Journal	FEBS Letters
Volume	510
Issue number	1-2
Pages (from-to)	41-4
Number of pages	4
ISSN	0014-5793
Publication status	Published - 2 Jan 2002

Keywords

Chloramines
Drug Synergism
Hypochlorous Acid
Proteins
Reactive Nitrogen Species
Serum Albumin, Bovine
Superoxides

Cite this

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title = "Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins",

abstract = "Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.",

keywords = "Chloramines, Drug Synergism, Hypochlorous Acid, Proteins, Reactive Nitrogen Species, Serum Albumin, Bovine, Superoxides",

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TY - JOUR

T1 - Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

AU - Hawkins, Clare Louise

AU - Rees, Martin D

AU - Davies, Michael Jonathan

PY - 2002/1/2

Y1 - 2002/1/2

N2 - Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.

AB - Activated phagocytes generate both superoxide radicals via a respiratory burst, and HOCl via the concurrent release of the haem enzyme myeloperoxidase. Amine and amide functions on proteins and carbohydrates are major targets for HOCl, generating chloramines (RNHCl) and chloramides (RC(O)NClR'), which can accumulate to high concentrations (>100 microM). Here we show that superoxide radicals catalyse the decomposition of chloramines and chloramides to reactive nitrogen-centred radicals, and increase the extent of protein fragmentation compared to that observed with either superoxide radicals or HOCl, alone. This synergistic action may be of significance at sites of inflammation, where both superoxide radicals and chloramines/chloramides are formed simultaneously.

KW - Chloramines

KW - Drug Synergism

KW - Hypochlorous Acid

KW - Proteins

KW - Reactive Nitrogen Species

KW - Serum Albumin, Bovine

KW - Superoxides

M3 - Journal article

C2 - 11755528

SN - 0014-5793

VL - 510

SP - 41

EP - 44

JO - F E B S Letters

JF - F E B S Letters

IS - 1-2

ER -

Superoxide radicals can act synergistically with hypochlorite to induce damage to proteins

Abstract

Keywords

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