SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage

Ivo A Hendriks; Louise W Treffers; Matty Verlaan-de Vries; Jesper V Olsen; Alfred C O Vertegaal

doi:10.1016/j.celrep.2015.02.033

SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage

Ivo A Hendriks, Louise W Treffers, Matty Verlaan-de Vries, Jesper V Olsen, Alfred C O Vertegaal

78 Citations (Scopus)

Abstract

Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.

Original language	English
Journal	Cell Reports
Volume	10
Issue number	10
Pages (from-to)	1778–1791
ISSN	2211-1247
DOIs	https://doi.org/10.1016/j.celrep.2015.02.033
Publication status	Published - 17 Mar 2015

Access to Document

10.1016/j.celrep.2015.02.033

Cite this

@article{463113b948a748ab9f2209d0a9cc76cb,

title = "SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage",

abstract = "Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.",

author = "Hendriks, {Ivo A} and Treffers, {Louise W} and {Verlaan-de Vries}, Matty and Olsen, {Jesper V} and Vertegaal, {Alfred C O}",

year = "2015",

month = mar,

day = "17",

doi = "10.1016/j.celrep.2015.02.033",

language = "English",

volume = "10",

pages = " 1778–1791",

journal = "Cell Reports",

issn = "2639-1856",

publisher = "Cell Press",

number = "10",

}

TY - JOUR

T1 - SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage

AU - Hendriks, Ivo A

AU - Treffers, Louise W

AU - Verlaan-de Vries, Matty

AU - Olsen, Jesper V

AU - Vertegaal, Alfred C O

PY - 2015/3/17

Y1 - 2015/3/17

N2 - Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.

AB - Small ubiquitin-like modifiers play critical roles in the DNA damage response (DDR). To increase our understanding of SUMOylation in the mammalian DDR, we employed a quantitative proteomics approach in order to identify dynamically regulated SUMO-2 conjugates and modification sites upon treatment with the DNA damaging agent methyl methanesulfonate (MMS). We have uncovered a dynamic set of 20 upregulated and 33 downregulated SUMO-2 conjugates, and 755 SUMO-2 sites, of which 362 were dynamic in response to MMS. In contrast to yeast, where a response is centered on homologous recombination, we identified dynamically SUMOylated interaction networks of chromatin modifiers, transcription factors, DNA repair factors, and nuclear body components. SUMOylated chromatin modifiers include JARID1B/KDM5B, JARID1C/KDM5C, p300, CBP, PARP1, SetDB1, and MBD1. Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to demethylate histone H3K4.

U2 - 10.1016/j.celrep.2015.02.033

DO - 10.1016/j.celrep.2015.02.033

M3 - Journal article

C2 - 25772364

SN - 2639-1856

VL - 10

SP - 1778

EP - 1791

JO - Cell Reports

JF - Cell Reports

IS - 10

ER -

SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage

Abstract

Access to Document

Fingerprint

Cite this