Subretinal Implantation of Electrospun, Short Nanowire, and Smooth Poly(e-caprolactone) Scaffolds to the Subretinal Space of Porcine Eyes

TY - JOUR

T1 - Subretinal Implantation of Electrospun, Short Nanowire, and Smooth Poly(e-caprolactone) Scaffolds to the Subretinal Space of Porcine Eyes

AU - Christiansen, A T

AU - Tao, Shanwen

AU - Smith, M

AU - Wnek, G E

AU - Prause, J U

AU - Young, M J

AU - Klassen, H

AU - Kaplan, H J

AU - la Cour, Mette Funding

AU - Kiilgaard, J F

PY - 2012/1/4

Y1 - 2012/1/4

N2 - Biodegradable scaffolds play an important adjunct role in transplantation of retinal progenitor cells (RPCs) to the subretinal space. Poly(μ- Caprolactone) (PCL) scaffolds with different modifications were subretinally implanted in 28 porcine eyes and evaluated by multifocal electroretinography (mfERG) and histology after 6 weeks of observation. PCL Short Nanowire, PCL Electrospun, and PCL Smooth scaffolds were well tolerated in the subretinal space in pigs and caused no inflammation and limited tissue disruption. PCL Short Nanowire had an average rate of preserved overlying outer retina 17 higher than PCL Electrospun and 25 higher than PCL Smooth. Furthermore, PCL Short Nanowire was found to have the most suitable degree of stiffness for surgical delivery to the subretinal space. The membrane-induced photoreceptor damage could be shown on mfERG, but the reductions in P1 amplitude were only significant for the PCL Smooth. We conclude that of the tested scaffolds, PCL Short Nanowire is the best candidate for subretinal implantation.

AB - Biodegradable scaffolds play an important adjunct role in transplantation of retinal progenitor cells (RPCs) to the subretinal space. Poly(μ- Caprolactone) (PCL) scaffolds with different modifications were subretinally implanted in 28 porcine eyes and evaluated by multifocal electroretinography (mfERG) and histology after 6 weeks of observation. PCL Short Nanowire, PCL Electrospun, and PCL Smooth scaffolds were well tolerated in the subretinal space in pigs and caused no inflammation and limited tissue disruption. PCL Short Nanowire had an average rate of preserved overlying outer retina 17 higher than PCL Electrospun and 25 higher than PCL Smooth. Furthermore, PCL Short Nanowire was found to have the most suitable degree of stiffness for surgical delivery to the subretinal space. The membrane-induced photoreceptor damage could be shown on mfERG, but the reductions in P1 amplitude were only significant for the PCL Smooth. We conclude that of the tested scaffolds, PCL Short Nanowire is the best candidate for subretinal implantation.

U2 - 10.1155/2012/454295

DO - 10.1155/2012/454295

M3 - Journal article

C2 - 22550509

SN - 1687-966X

VL - 2012

SP - 1

EP - 8

JO - Stem Cells International

JF - Stem Cells International

IS - 454295

ER -