Subcellular localization and mechanism of secretion of vascular endothelial growth factor in human skeletal muscle

TY - JOUR

T1 - Subcellular localization and mechanism of secretion of vascular endothelial growth factor in human skeletal muscle

AU - Høier, Birgitte

AU - Prats Gavalda, Clara

AU - Qvortrup, Klaus

AU - Pilegaard, Henriette

AU - Bangsbo, Jens

AU - Hellsten, Ylva

N1 - CURIS 2013 NEXS 123

PY - 2013/9

Y1 - 2013/9

N2 - The subcellular distribution and secretion of vascular endothelial growth factor (VEGF) was examined in skeletal muscle of healthy humans. Skeletal muscle biopsies were obtained from m.v. lateralis before and after a 2 h bout of cycling exercise. VEGF localization was conducted on preparations of teased muscle fibers by transmission electron microscopy (TEM) and confocal microscopy (CM). Muscle interstitial fluid was sampled from microdialysis probes placed in the thigh muscle. TEM and CM analysis revealed two primary sites of localization of VEGF: in vesicles located in the subsarcolemmal regions and between the contractile elements within the muscle fibers; and in pericytes situated on the skeletal muscle capillaries. Quantitation of the subsarcolemmal density of VEGF vesicles, calculated on top of myonuclei, in the muscle fibers revealed a ∼50% increase (P

AB - The subcellular distribution and secretion of vascular endothelial growth factor (VEGF) was examined in skeletal muscle of healthy humans. Skeletal muscle biopsies were obtained from m.v. lateralis before and after a 2 h bout of cycling exercise. VEGF localization was conducted on preparations of teased muscle fibers by transmission electron microscopy (TEM) and confocal microscopy (CM). Muscle interstitial fluid was sampled from microdialysis probes placed in the thigh muscle. TEM and CM analysis revealed two primary sites of localization of VEGF: in vesicles located in the subsarcolemmal regions and between the contractile elements within the muscle fibers; and in pericytes situated on the skeletal muscle capillaries. Quantitation of the subsarcolemmal density of VEGF vesicles, calculated on top of myonuclei, in the muscle fibers revealed a ∼50% increase (P

U2 - 10.1096/fj.12-224618

DO - 10.1096/fj.12-224618

M3 - Journal article

C2 - 23709615

SN - 0892-6638

VL - 27

SP - 3496

EP - 3504

JO - F A S E B Journal

JF - F A S E B Journal

IS - 9

ER -