TY - JOUR
T1 - Study of endophytic Xylariaceae in Thailand: diversity and taxonomy inferred from rDNA sequence analyses with saprobes forming fruit bodies in the field
AU - Okane, Izumi
AU - Srikitikulchai, Prasert
AU - Toyama, Kyoko
AU - Læssøe, Thomas
AU - Sivichai, Somsak
AU - Hywell-Jones, Nigel
AU - Nakagiri, Akira
AU - Potacharoen, Wanchern
AU - Suzuki, Ken-ichiro
N1 - Keywords: Diversity, Endophytic-saprobic relationships, rDNA sequence analysis, Thailand, Xylariaceous endophytes
PY - 2008
Y1 - 2008
N2 - A study of the diversity, taxonomy, and ecology of endophytic Xylariaceae (Ascomycota) was carried out. In this study, we obtained isolates of Xylariaceae from healthy, attached leaves and teleomorphic stromata on decayed plant materials in a permanent plot at Khao Yai National Park (Thailand). In addition, strains deposited beforehand were selected in which both endophytic strains isolated from living plant tissues and saprobic strains from fruit bodies were included. Consequently, 405 strains of Xylariaceae (273 endophytic and 132 saprobic strains, including identified strains) were studied to reveal the diversity and taxonomy of endophytes and the relationships between those endophytes and saprobic Xylariaceae in Thailand that have been recorded according to fruit-body formation on decayed plant materials. Analysis of 28S rDNA D1/D2 sequences revealed 21 xylariaceous species inhabiting tropical foliage at the site, and several species that are already known as saprobes appear to be among those isolated from living leaves. Furthermore, several clades that consisted of only endophytic strains were found, and some of these have no known matches in public DNA sequence banks.
AB - A study of the diversity, taxonomy, and ecology of endophytic Xylariaceae (Ascomycota) was carried out. In this study, we obtained isolates of Xylariaceae from healthy, attached leaves and teleomorphic stromata on decayed plant materials in a permanent plot at Khao Yai National Park (Thailand). In addition, strains deposited beforehand were selected in which both endophytic strains isolated from living plant tissues and saprobic strains from fruit bodies were included. Consequently, 405 strains of Xylariaceae (273 endophytic and 132 saprobic strains, including identified strains) were studied to reveal the diversity and taxonomy of endophytes and the relationships between those endophytes and saprobic Xylariaceae in Thailand that have been recorded according to fruit-body formation on decayed plant materials. Analysis of 28S rDNA D1/D2 sequences revealed 21 xylariaceous species inhabiting tropical foliage at the site, and several species that are already known as saprobes appear to be among those isolated from living leaves. Furthermore, several clades that consisted of only endophytic strains were found, and some of these have no known matches in public DNA sequence banks.
U2 - 10.1007/s10267-008-0440-6
DO - 10.1007/s10267-008-0440-6
M3 - Journal article
SN - 1340-3540
VL - 49
SP - 359
EP - 372
JO - Mycoscience
JF - Mycoscience
IS - 6
ER -