Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle X-ray scattering

    10 Citations (Scopus)

    Abstract

    Structural investigation of the species present during protein fibrillation is of tremendous importance, yet complicated by the equilibrium between species of very different sizes and life-times. Small-angle X-ray scattering may be applied to solve this problem, providing both information about the process (number of species present and volume fractions of individual species) and low-resolution three-dimensional shape reconstructions of individual species. Here, we describe in detail the challenges associated with the approach, exemplified using data from fibrillating insulin or α-synuclein samples.

    Original languageEnglish
    Title of host publicationAmyloid proteins : methods and protocols
    EditorsEinar M Sigurdsson, Miguel Calero, Maria Gasset
    Number of pages18
    Volume849
    Place of PublicationNew York
    PublisherSpringer Science+Business Media
    Publication date2012
    Edition2
    Pages137-155
    Chapter10
    ISBN (Print)978-1-61779-550-3
    ISBN (Electronic)978-1-61779-551-0
    DOIs
    Publication statusPublished - 2012
    SeriesMethods in Molecular Biology
    Volume849
    ISSN1064-3745

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