Structural and functional organization of the HF.10 human zinc finger gene (ZNF35) located on chromosome 3p21-p22.

TY - JOUR

T1 - Structural and functional organization of the HF.10 human zinc finger gene (ZNF35) located on chromosome 3p21-p22.

AU - Lanfrancone, L

AU - Pengue, G

AU - Pandolfi, P P

AU - Salcini, A E

AU - Giacomucci, A

AU - Longo, L

AU - Donti, E

AU - De Luca, P

AU - La Mantia, G

AU - Pelicci, P G

N1 - Keywords: Amino Acid Sequence; Base Sequence; Chromosome Mapping; Chromosomes, Human, Pair 3; DNA; Genes; Humans; Molecular Sequence Data; Pseudogenes; Trans-Activators; Transcription, Genetic; Zinc Fingers

PY - 1992

Y1 - 1992

N2 - We report the structural and functional characterization of the HF.10 zinc finger gene (ZNF35) in normal human cells, as well as a processed pseudogene. The HF.10 gene spans about 13 kb and it is interrupted by three introns. All 11 zinc finger DNA-binding domains are contiguously encoded within the last 3' exon. The genomic region surrounding HF.10 exon 1 contains a CpG island and acts as a promoter in vitro. Using transient CAT assay in cotransfection experiments in cultured cells, we have determined that the HF.10 finger protein is a transcriptional transactivator. Restriction enzyme mapping and partial nucleotide sequencing of the HF.10 pseudogene indicated that it has arisen by retroposition of spliced HF.10 mRNA. In situ hybridization experiments revealed that both the functional locus and the pseudogene map to chromosome 3p21p22, a region that is frequently deleted in small cell lung and renal carcinomas. Hybridization of the HF.10 gene and the HF.10 pseudogene DNA probes to metaphases from a small cell lung carcinoma cell line with the 3p deletion revealed that both loci are part of the deleted chromosome region.

AB - We report the structural and functional characterization of the HF.10 zinc finger gene (ZNF35) in normal human cells, as well as a processed pseudogene. The HF.10 gene spans about 13 kb and it is interrupted by three introns. All 11 zinc finger DNA-binding domains are contiguously encoded within the last 3' exon. The genomic region surrounding HF.10 exon 1 contains a CpG island and acts as a promoter in vitro. Using transient CAT assay in cotransfection experiments in cultured cells, we have determined that the HF.10 finger protein is a transcriptional transactivator. Restriction enzyme mapping and partial nucleotide sequencing of the HF.10 pseudogene indicated that it has arisen by retroposition of spliced HF.10 mRNA. In situ hybridization experiments revealed that both the functional locus and the pseudogene map to chromosome 3p21p22, a region that is frequently deleted in small cell lung and renal carcinomas. Hybridization of the HF.10 gene and the HF.10 pseudogene DNA probes to metaphases from a small cell lung carcinoma cell line with the 3p deletion revealed that both loci are part of the deleted chromosome region.

M3 - Journal article

C2 - 1572646

SN - 0888-7543

VL - 12

SP - 720

EP - 728

JO - Genomics

JF - Genomics

IS - 4

ER -