TY - JOUR
T1 - Strategies for the capillary electrophoretic separation of indole alkaloids in Psilocybe semilanceata
AU - Pedersen-Bjergaard, Stig
AU - Rasmussen, Knut Einar
AU - Sannes, Ellen
PY - 1998/1
Y1 - 1998/1
N2 - While the hallucinogenic mushrooms Psilocybe semilanceata have previously been analyzed for the indole alkaloids psilocybin and baeocystin by capillary zone electrophoresis (CZE) at pH 11.5, the present work focused on the development of an alternative and complementary capillary electrophoretic method for their identification. Owing to their structural similarity and zwitterionic nature, the compounds were difficult to resolve based on different interactions with cationic or anionic micelles. However, while the attempts with micellar electrokinetic chromatography (MEKC) were unsuccessful, rapid derivatization with propyl chloroformate and reanalysis by CZE at pH 11.5 was effective to support identification of the two indole alkaloids. Psilocin was difficult to analyze by CZE at pH 11.5 owing to comigration with the electroosmotic flow. For this compound, the pH of the running buffer was reduced to 7.2 to effectively enhance the electrophoretic mobility.
AB - While the hallucinogenic mushrooms Psilocybe semilanceata have previously been analyzed for the indole alkaloids psilocybin and baeocystin by capillary zone electrophoresis (CZE) at pH 11.5, the present work focused on the development of an alternative and complementary capillary electrophoretic method for their identification. Owing to their structural similarity and zwitterionic nature, the compounds were difficult to resolve based on different interactions with cationic or anionic micelles. However, while the attempts with micellar electrokinetic chromatography (MEKC) were unsuccessful, rapid derivatization with propyl chloroformate and reanalysis by CZE at pH 11.5 was effective to support identification of the two indole alkaloids. Psilocin was difficult to analyze by CZE at pH 11.5 owing to comigration with the electroosmotic flow. For this compound, the pH of the running buffer was reduced to 7.2 to effectively enhance the electrophoretic mobility.
KW - Capillary electrophoresis
KW - Indole alkaloids
KW - Psilocybe semilanceata
UR - http://www.scopus.com/inward/record.url?scp=0031931248&partnerID=8YFLogxK
U2 - 10.1002/elps.1150190107
DO - 10.1002/elps.1150190107
M3 - Journal article
C2 - 9511859
AN - SCOPUS:0031931248
SN - 0173-0835
VL - 19
SP - 27
EP - 30
JO - Electrophoresis
JF - Electrophoresis
IS - 1
ER -