Storage of oral fluid as dried spots on alginate and chitosan foam – A new concept for oral fluid collection

Christine Pernille Lødøen; Lars Erik  Eng Eibak; Knut Einar  Rasmussen; Stig Pedersen-Bjergaard; Therese  Andersen; Astrid  Gjelstad

Storage of oral fluid as dried spots on alginate and chitosan foam – A new concept for oral fluid collection

Christine Pernille Lødøen, Lars Erik Eng Eibak, Knut Einar Rasmussen, Stig Pedersen-Bjergaard, Therese Andersen, Astrid Gjelstad

21 Citations (Scopus)

Abstract

Background: In recent years, there has been a major focus on analyzing drug substances from dried μl volumes of biological fluids, commonly known as dried matrix spotting. Results: 10 μl oral fluid, spiked with five basic drugs, was collected and stored as dried spots on alginate and chitosan foam, and subsequently dissolved; the drugs were thereafter isolated with electromembrane extraction and analyzed by LC-MS. The correlation coefficients were above 0.9885 for all drugs in the range 25-1000 ng/ml. The reported RSD values were below 15%. Conclusion: Storage of oral fluid as dried spots on alginate and chitosan foam have been investigated for the first time. The extract obtained after electromembrane extraction was directly compatible with LC-MS, and apparently free from coextracted matrix components.

Original language	English
Journal	Bioanalysis
Volume	3
Pages (from-to)	317-325
Number of pages	9
ISSN	1757-6180
Publication status	Published - Feb 2013

Cite this

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title = "Storage of oral fluid as dried spots on alginate and chitosan foam – A new concept for oral fluid collection",

abstract = "Background: In recent years, there has been a major focus on analyzing drug substances from dried μl volumes of biological fluids, commonly known as dried matrix spotting. Results: 10 μl oral fluid, spiked with five basic drugs, was collected and stored as dried spots on alginate and chitosan foam, and subsequently dissolved; the drugs were thereafter isolated with electromembrane extraction and analyzed by LC-MS. The correlation coefficients were above 0.9885 for all drugs in the range 25-1000 ng/ml. The reported RSD values were below 15%. Conclusion: Storage of oral fluid as dried spots on alginate and chitosan foam have been investigated for the first time. The extract obtained after electromembrane extraction was directly compatible with LC-MS, and apparently free from coextracted matrix components.",

author = "L{\o}d{\o}en, {Christine Pernille} and {Eng Eibak}, {Lars Erik} and Rasmussen, {Knut Einar} and Stig Pedersen-Bjergaard and Therese Andersen and Astrid Gjelstad",

year = "2013",

month = feb,

language = "English",

volume = "3",

pages = "317--325",

journal = "Bioanalysis",

issn = "1757-6180",

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TY - JOUR

T1 - Storage of oral fluid as dried spots on alginate and chitosan foam – A new concept for oral fluid collection

AU - Lødøen, Christine Pernille

AU - Eng Eibak, Lars Erik

AU - Rasmussen, Knut Einar

AU - Pedersen-Bjergaard, Stig

AU - Andersen, Therese

AU - Gjelstad, Astrid

PY - 2013/2

Y1 - 2013/2

N2 - Background: In recent years, there has been a major focus on analyzing drug substances from dried μl volumes of biological fluids, commonly known as dried matrix spotting. Results: 10 μl oral fluid, spiked with five basic drugs, was collected and stored as dried spots on alginate and chitosan foam, and subsequently dissolved; the drugs were thereafter isolated with electromembrane extraction and analyzed by LC-MS. The correlation coefficients were above 0.9885 for all drugs in the range 25-1000 ng/ml. The reported RSD values were below 15%. Conclusion: Storage of oral fluid as dried spots on alginate and chitosan foam have been investigated for the first time. The extract obtained after electromembrane extraction was directly compatible with LC-MS, and apparently free from coextracted matrix components.

AB - Background: In recent years, there has been a major focus on analyzing drug substances from dried μl volumes of biological fluids, commonly known as dried matrix spotting. Results: 10 μl oral fluid, spiked with five basic drugs, was collected and stored as dried spots on alginate and chitosan foam, and subsequently dissolved; the drugs were thereafter isolated with electromembrane extraction and analyzed by LC-MS. The correlation coefficients were above 0.9885 for all drugs in the range 25-1000 ng/ml. The reported RSD values were below 15%. Conclusion: Storage of oral fluid as dried spots on alginate and chitosan foam have been investigated for the first time. The extract obtained after electromembrane extraction was directly compatible with LC-MS, and apparently free from coextracted matrix components.

M3 - Journal article

SN - 1757-6180

VL - 3

SP - 317

EP - 325

JO - Bioanalysis

JF - Bioanalysis

ER -

Storage of oral fluid as dried spots on alginate and chitosan foam – A new concept for oral fluid collection

Abstract

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