Stereomicroscopic examination of stained rectal biopsies

S. S. Poulsen; K. C. Christensen; M. Petri; S. Jarnum

doi:10.3109/00365527809181771

Stereomicroscopic examination of stained rectal biopsies

S. S. Poulsen^*, K. C. Christensen, M. Petri, S. Jarnum

^*Corresponding author for this work

Endocrinology and Metabolism

10 Citations (Scopus)

Abstract

The normal mucosal surface morphology in the rectum has been described by investigation of 15 rectal biopsies using a semimacroscopic technique. The structures of the mucosal surface were rendered visible by staining the biopsies as whole mounts with Alcian Green. A regular pattern of the crypt openings was found, and the goblet cells were observed in and just around the crypt openings. The density of crypt openings was calculated as the number of crypt openings per square millimetre (104 ± 23 openings per mm², mean and S.D.). Histologic examinations of the same biopsies were performed, and the density of crypt tubules was calculated from serial, horizontal sections (130 ± 34 tubules per mm², mean and S.D.). The ratio of crypt openings/crypt tubules was 1:1.25, indicating some branching of the mucosal glands in the rectum.

Original language	English
Book series	Scandinavian Journal of Gastroenterology
Volume	13
Issue number	5
Pages (from-to)	605-608
Number of pages	4
ISSN	0036-5521
DOIs	https://doi.org/10.3109/00365527809181771
Publication status	Published - 1 Jan 1978

Keywords

Biopsy
Histology
Humans
Rectum
Stereomicroscopy

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10.3109/00365527809181771

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title = "Stereomicroscopic examination of stained rectal biopsies",

abstract = "The normal mucosal surface morphology in the rectum has been described by investigation of 15 rectal biopsies using a semimacroscopic technique. The structures of the mucosal surface were rendered visible by staining the biopsies as whole mounts with Alcian Green. A regular pattern of the crypt openings was found, and the goblet cells were observed in and just around the crypt openings. The density of crypt openings was calculated as the number of crypt openings per square millimetre (104 ± 23 openings per mm2, mean and S.D.). Histologic examinations of the same biopsies were performed, and the density of crypt tubules was calculated from serial, horizontal sections (130 ± 34 tubules per mm2, mean and S.D.). The ratio of crypt openings/crypt tubules was 1:1.25, indicating some branching of the mucosal glands in the rectum.",

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T1 - Stereomicroscopic examination of stained rectal biopsies

AU - Poulsen, S. S.

AU - Christensen, K. C.

AU - Petri, M.

AU - Jarnum, S.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - The normal mucosal surface morphology in the rectum has been described by investigation of 15 rectal biopsies using a semimacroscopic technique. The structures of the mucosal surface were rendered visible by staining the biopsies as whole mounts with Alcian Green. A regular pattern of the crypt openings was found, and the goblet cells were observed in and just around the crypt openings. The density of crypt openings was calculated as the number of crypt openings per square millimetre (104 ± 23 openings per mm2, mean and S.D.). Histologic examinations of the same biopsies were performed, and the density of crypt tubules was calculated from serial, horizontal sections (130 ± 34 tubules per mm2, mean and S.D.). The ratio of crypt openings/crypt tubules was 1:1.25, indicating some branching of the mucosal glands in the rectum.

AB - The normal mucosal surface morphology in the rectum has been described by investigation of 15 rectal biopsies using a semimacroscopic technique. The structures of the mucosal surface were rendered visible by staining the biopsies as whole mounts with Alcian Green. A regular pattern of the crypt openings was found, and the goblet cells were observed in and just around the crypt openings. The density of crypt openings was calculated as the number of crypt openings per square millimetre (104 ± 23 openings per mm2, mean and S.D.). Histologic examinations of the same biopsies were performed, and the density of crypt tubules was calculated from serial, horizontal sections (130 ± 34 tubules per mm2, mean and S.D.). The ratio of crypt openings/crypt tubules was 1:1.25, indicating some branching of the mucosal glands in the rectum.

KW - Biopsy

KW - Histology

KW - Humans

KW - Rectum

KW - Stereomicroscopy

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JF - Scandinavian Journal of Gastroenterology. Supplement

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Stereomicroscopic examination of stained rectal biopsies

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Keywords

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