Statistical Approaches for DNA Barcoding

TY - JOUR

T1 - Statistical Approaches for DNA Barcoding

AU - Nielsen, Rasmus

AU - Matz, M.

PY - 2006

Y1 - 2006

N2 - The use of DNA as a tool for species identification has become known as "DNA barcoding" (Floyd et al., 2002; Hebert et al., 2003; Remigio and Hebert, 2003). The basic idea is straightforward: a small amount of DNA is extracted from the specimen, amplified and sequenced. The gene region sequenced is chosen so that it is nearly identical among individuals of the same species, but different between species, and therefore its sequence, can serve as an identification tag for the species ("DNA barcode"). By matching the sequence obtained from an unidentified specimen ("query" sequence) to the database of sequences from known species, one can thus determine the species affiliation of the specimen. Importantly, the specimen may represent any developmental stage or be just a small fragment of the whole organism, displaying no morphological traits required for standard identification. Although this technique will by no means eliminate

AB - The use of DNA as a tool for species identification has become known as "DNA barcoding" (Floyd et al., 2002; Hebert et al., 2003; Remigio and Hebert, 2003). The basic idea is straightforward: a small amount of DNA is extracted from the specimen, amplified and sequenced. The gene region sequenced is chosen so that it is nearly identical among individuals of the same species, but different between species, and therefore its sequence, can serve as an identification tag for the species ("DNA barcode"). By matching the sequence obtained from an unidentified specimen ("query" sequence) to the database of sequences from known species, one can thus determine the species affiliation of the specimen. Importantly, the specimen may represent any developmental stage or be just a small fragment of the whole organism, displaying no morphological traits required for standard identification. Although this technique will by no means eliminate

U2 - 10.1080/10635150500431239

DO - 10.1080/10635150500431239

M3 - Journal article

SN - 1063-5157

VL - 55

SP - 162

EP - 169

JO - Systematic Biology

JF - Systematic Biology

IS - 1

ER -