Staphylococcus agnetis, a potential pathogen in broiler breeders

Louise Ladefoged Poulsen; Ida Thøfner; Magne Bisgaard; Rikke Heidemann Olsen; Jens Peter Christensen; Henrik Christensen

doi:10.1016/j.vetmic.2017.10.018

Staphylococcus agnetis, a potential pathogen in broiler breeders

Louise Ladefoged Poulsen^*, Ida Thøfner, Magne Bisgaard, Rikke Heidemann Olsen, Jens Peter Christensen, Henrik Christensen

^*Corresponding author for this work

10 Citations (Scopus)

Abstract

In this study, four broiler parent flocks have been followed from the onset of the production period (week 20) until slaughter (week 60). Every week, approximately ten dead broiler breeders, randomly selected among birds dead on their own, were collected and subjected to a full post mortem analysis including bacteriological examination. In total 997 breeders were investigated and for the first time Staphylococcus agnetis was isolated in pure culture from cases of endocarditis and septicemia from 16 broiler breeders. In addition, the cloacal flora from newly hatched chickens originating from the same four flocks were characterized and S. agnetis was found in pure culture of several newly hatched chickens (n = 12) and only in one case in combination with another species. Clonality of the isolates was examined by pulsed-field-gel-electrophoresis which showed indistinguishable patterns in isolates from both broiler breeders and broilers. Three isolates were whole genome sequenced to obtain knowledge on virulence genes. The isolates harbored a number of genes encoding different fibrinogen binding proteins and toxins which might be important for virulence. The present findings demonstrate that S. agnetis may be associated with mortality in broiler breeders. No disease was associated with the broilers which were found positive for S. agnetis in the cloaca.

Original language	English
Journal	Veterinary Microbiology
Volume	212
Pages (from-to)	1-6
Number of pages	6
ISSN	0378-1135
DOIs	https://doi.org/10.1016/j.vetmic.2017.10.018
Publication status	Published - 2017

Keywords

Disease
Endocarditis
Pathogen
Poultry
Septicaemia

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10.1016/j.vetmic.2017.10.018

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title = "Staphylococcus agnetis, a potential pathogen in broiler breeders",

abstract = "In this study, four broiler parent flocks have been followed from the onset of the production period (week 20) until slaughter (week 60). Every week, approximately ten dead broiler breeders, randomly selected among birds dead on their own, were collected and subjected to a full post mortem analysis including bacteriological examination. In total 997 breeders were investigated and for the first time Staphylococcus agnetis was isolated in pure culture from cases of endocarditis and septicemia from 16 broiler breeders. In addition, the cloacal flora from newly hatched chickens originating from the same four flocks were characterized and S. agnetis was found in pure culture of several newly hatched chickens (n = 12) and only in one case in combination with another species. Clonality of the isolates was examined by pulsed-field-gel-electrophoresis which showed indistinguishable patterns in isolates from both broiler breeders and broilers. Three isolates were whole genome sequenced to obtain knowledge on virulence genes. The isolates harbored a number of genes encoding different fibrinogen binding proteins and toxins which might be important for virulence. The present findings demonstrate that S. agnetis may be associated with mortality in broiler breeders. No disease was associated with the broilers which were found positive for S. agnetis in the cloaca.",

keywords = "Disease, Endocarditis, Pathogen, Poultry, Septicaemia",

author = "Poulsen, {Louise Ladefoged} and Ida Th{\o}fner and Magne Bisgaard and Olsen, {Rikke Heidemann} and Christensen, {Jens Peter} and Henrik Christensen",

year = "2017",

doi = "10.1016/j.vetmic.2017.10.018",

language = "English",

volume = "212",

pages = "1--6",

journal = "Veterinary Microbiology",

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TY - JOUR

T1 - Staphylococcus agnetis, a potential pathogen in broiler breeders

AU - Poulsen, Louise Ladefoged

AU - Thøfner, Ida

AU - Bisgaard, Magne

AU - Olsen, Rikke Heidemann

AU - Christensen, Jens Peter

AU - Christensen, Henrik

PY - 2017

Y1 - 2017

N2 - In this study, four broiler parent flocks have been followed from the onset of the production period (week 20) until slaughter (week 60). Every week, approximately ten dead broiler breeders, randomly selected among birds dead on their own, were collected and subjected to a full post mortem analysis including bacteriological examination. In total 997 breeders were investigated and for the first time Staphylococcus agnetis was isolated in pure culture from cases of endocarditis and septicemia from 16 broiler breeders. In addition, the cloacal flora from newly hatched chickens originating from the same four flocks were characterized and S. agnetis was found in pure culture of several newly hatched chickens (n = 12) and only in one case in combination with another species. Clonality of the isolates was examined by pulsed-field-gel-electrophoresis which showed indistinguishable patterns in isolates from both broiler breeders and broilers. Three isolates were whole genome sequenced to obtain knowledge on virulence genes. The isolates harbored a number of genes encoding different fibrinogen binding proteins and toxins which might be important for virulence. The present findings demonstrate that S. agnetis may be associated with mortality in broiler breeders. No disease was associated with the broilers which were found positive for S. agnetis in the cloaca.

AB - In this study, four broiler parent flocks have been followed from the onset of the production period (week 20) until slaughter (week 60). Every week, approximately ten dead broiler breeders, randomly selected among birds dead on their own, were collected and subjected to a full post mortem analysis including bacteriological examination. In total 997 breeders were investigated and for the first time Staphylococcus agnetis was isolated in pure culture from cases of endocarditis and septicemia from 16 broiler breeders. In addition, the cloacal flora from newly hatched chickens originating from the same four flocks were characterized and S. agnetis was found in pure culture of several newly hatched chickens (n = 12) and only in one case in combination with another species. Clonality of the isolates was examined by pulsed-field-gel-electrophoresis which showed indistinguishable patterns in isolates from both broiler breeders and broilers. Three isolates were whole genome sequenced to obtain knowledge on virulence genes. The isolates harbored a number of genes encoding different fibrinogen binding proteins and toxins which might be important for virulence. The present findings demonstrate that S. agnetis may be associated with mortality in broiler breeders. No disease was associated with the broilers which were found positive for S. agnetis in the cloaca.

KW - Disease

KW - Endocarditis

KW - Pathogen

KW - Poultry

KW - Septicaemia

U2 - 10.1016/j.vetmic.2017.10.018

DO - 10.1016/j.vetmic.2017.10.018

M3 - Journal article

C2 - 29173582

AN - SCOPUS:85032304846

SN - 0378-1135

VL - 212

SP - 1

EP - 6

JO - Veterinary Microbiology

JF - Veterinary Microbiology

ER -

Staphylococcus agnetis, a potential pathogen in broiler breeders

Abstract

Keywords

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