Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent

Camilla Knudsen; Krutika Bavishi; Ketil Mathiasen Viborg; Damian Paul Drew; Henrik Toft Simonsen; Mohammed Saddik Motawia; Birger Lindberg Møller; Tomas Laursen

doi:10.1016/j.phytochem.2019.112214

Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent

Camilla Knudsen, Krutika Bavishi, Ketil Mathiasen Viborg, Damian Paul Drew, Henrik Toft Simonsen, Mohammed Saddik Motawia, Birger Lindberg Møller, Tomas Laursen

Section for Plant Biochemistry

6 Citations (Scopus)

Abstract

In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.

Original language	English
Journal	Phytochemistry
Volume	170
ISSN	0031-9422
DOIs	https://doi.org/10.1016/j.phytochem.2019.112214
Publication status	Published - Feb 2020

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Cite this

@article{265dc06cb9d945b883f174faf0186ea9,

title = "Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent",

abstract = "In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.",

keywords = "Sorghum bicolor (L.) Moench, Poaceae, Natural deep eutectic solvents, Metabolons, Cytochromes P450, Specialized metabolites, Liquid-liquid phase separation",

author = "Camilla Knudsen and Krutika Bavishi and Viborg, {Ketil Mathiasen} and Drew, {Damian Paul} and Simonsen, {Henrik Toft} and Motawia, {Mohammed Saddik} and M{\o}ller, {Birger Lindberg} and Tomas Laursen",

year = "2020",

month = feb,

doi = "10.1016/j.phytochem.2019.112214",

language = "English",

volume = "170",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Pergamon Press",

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TY - JOUR

T1 - Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent

AU - Knudsen, Camilla

AU - Bavishi, Krutika

AU - Viborg, Ketil Mathiasen

AU - Drew, Damian Paul

AU - Simonsen, Henrik Toft

AU - Motawia, Mohammed Saddik

AU - Møller, Birger Lindberg

AU - Laursen, Tomas

PY - 2020/2

Y1 - 2020/2

N2 - In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.

AB - In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.

KW - Sorghum bicolor (L.) Moench

KW - Poaceae

KW - Natural deep eutectic solvents

KW - Metabolons

KW - Cytochromes P450

KW - Specialized metabolites

KW - Liquid-liquid phase separation

U2 - 10.1016/j.phytochem.2019.112214

DO - 10.1016/j.phytochem.2019.112214

M3 - Journal article

C2 - 31794881

SN - 0031-9422

VL - 170

JO - Phytochemistry

JF - Phytochemistry

ER -