Spray dried cubosomes with ovalbumin and Quil-A as a nanoparticulate dry powder vaccine formulation

Christoffer von Halling Laier; Blake Gibson; Marco van de Weert; Ben J Boyd; Thomas Rades; Anja Boisen; Sarah Hook; Line Hagner Nielsen

doi:10.1016/j.ijpharm.2018.08.036

Spray dried cubosomes with ovalbumin and Quil-A as a nanoparticulate dry powder vaccine formulation

Christoffer von Halling Laier, Blake Gibson, Marco van de Weert, Ben J Boyd, Thomas Rades, Anja Boisen, Sarah Hook, Line Hagner Nielsen

9 Citations (Scopus)

Abstract

Subunit vaccine formulations are often produced as liquid dispersions through complicated processes. It is desirable, however, to have simple, cheap and up-scalable methods to produce nanoparticulate subunit vaccines in powder form. Here, a simple single-step spray drying process for production of powder cubosome precursors with the model antigen ovalbumin (OVA) and the adjuvant Quil-A is presented. The cubosomes were characterized in vitro and evaluated in vivo by subcutaneous and oral administration for their potential as a vaccine formulation. Hydrated cubosomes had average particle size of 257 ± 8 nm and zeta potential of −18.0 ± 0.6 mV. The powder contained 10.6 ± 0.7% w/w OVA prior to hydration, of which 65 ± 1% was released within the first 20 min in 9.5 mM PBS at pH 7.3, with the remaining OVA gradually released over the following 24 h. Immunization with cubosomes resulted in significantly stronger antigen-specific serum IgG responses (p < 0.01), CD8⁺ T cell expansion (p < 0.0001) and target T cell killing compared to controls when given s.c., and was ineffective orally. This study shows that spray drying is a suitable method for producing nanoparticulate vaccine formulations in dry powder form.

Original language	English
Journal	International Journal of Pharmaceutics
Volume	550
Issue number	1-2
Pages (from-to)	35-44
Number of pages	10
ISSN	0378-5173
DOIs	https://doi.org/10.1016/j.ijpharm.2018.08.036
Publication status	Published - 25 Oct 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijpharm.2018.08.036

Cite this

@article{72894e76938f4ea086052d16dc0f99c0,

title = "Spray dried cubosomes with ovalbumin and Quil-A as a nanoparticulate dry powder vaccine formulation",

abstract = "Subunit vaccine formulations are often produced as liquid dispersions through complicated processes. It is desirable, however, to have simple, cheap and up-scalable methods to produce nanoparticulate subunit vaccines in powder form. Here, a simple single-step spray drying process for production of powder cubosome precursors with the model antigen ovalbumin (OVA) and the adjuvant Quil-A is presented. The cubosomes were characterized in vitro and evaluated in vivo by subcutaneous and oral administration for their potential as a vaccine formulation. Hydrated cubosomes had average particle size of 257 ± 8 nm and zeta potential of −18.0 ± 0.6 mV. The powder contained 10.6 ± 0.7% w/w OVA prior to hydration, of which 65 ± 1% was released within the first 20 min in 9.5 mM PBS at pH 7.3, with the remaining OVA gradually released over the following 24 h. Immunization with cubosomes resulted in significantly stronger antigen-specific serum IgG responses (p < 0.01), CD8+ T cell expansion (p < 0.0001) and target T cell killing compared to controls when given s.c., and was ineffective orally. This study shows that spray drying is a suitable method for producing nanoparticulate vaccine formulations in dry powder form.",

author = "{von Halling Laier}, Christoffer and Blake Gibson and {van de Weert}, Marco and Boyd, {Ben J} and Thomas Rades and Anja Boisen and Sarah Hook and Nielsen, {Line Hagner}",

year = "2018",

month = oct,

day = "25",

doi = "10.1016/j.ijpharm.2018.08.036",

language = "English",

volume = "550",

pages = "35--44",

journal = "International Journal of Pharmaceutics",

issn = "0378-5173",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Spray dried cubosomes with ovalbumin and Quil-A as a nanoparticulate dry powder vaccine formulation

AU - von Halling Laier, Christoffer

AU - Gibson, Blake

AU - van de Weert, Marco

AU - Boyd, Ben J

AU - Rades, Thomas

AU - Boisen, Anja

AU - Hook, Sarah

AU - Nielsen, Line Hagner

PY - 2018/10/25

Y1 - 2018/10/25

N2 - Subunit vaccine formulations are often produced as liquid dispersions through complicated processes. It is desirable, however, to have simple, cheap and up-scalable methods to produce nanoparticulate subunit vaccines in powder form. Here, a simple single-step spray drying process for production of powder cubosome precursors with the model antigen ovalbumin (OVA) and the adjuvant Quil-A is presented. The cubosomes were characterized in vitro and evaluated in vivo by subcutaneous and oral administration for their potential as a vaccine formulation. Hydrated cubosomes had average particle size of 257 ± 8 nm and zeta potential of −18.0 ± 0.6 mV. The powder contained 10.6 ± 0.7% w/w OVA prior to hydration, of which 65 ± 1% was released within the first 20 min in 9.5 mM PBS at pH 7.3, with the remaining OVA gradually released over the following 24 h. Immunization with cubosomes resulted in significantly stronger antigen-specific serum IgG responses (p < 0.01), CD8+ T cell expansion (p < 0.0001) and target T cell killing compared to controls when given s.c., and was ineffective orally. This study shows that spray drying is a suitable method for producing nanoparticulate vaccine formulations in dry powder form.

AB - Subunit vaccine formulations are often produced as liquid dispersions through complicated processes. It is desirable, however, to have simple, cheap and up-scalable methods to produce nanoparticulate subunit vaccines in powder form. Here, a simple single-step spray drying process for production of powder cubosome precursors with the model antigen ovalbumin (OVA) and the adjuvant Quil-A is presented. The cubosomes were characterized in vitro and evaluated in vivo by subcutaneous and oral administration for their potential as a vaccine formulation. Hydrated cubosomes had average particle size of 257 ± 8 nm and zeta potential of −18.0 ± 0.6 mV. The powder contained 10.6 ± 0.7% w/w OVA prior to hydration, of which 65 ± 1% was released within the first 20 min in 9.5 mM PBS at pH 7.3, with the remaining OVA gradually released over the following 24 h. Immunization with cubosomes resulted in significantly stronger antigen-specific serum IgG responses (p < 0.01), CD8+ T cell expansion (p < 0.0001) and target T cell killing compared to controls when given s.c., and was ineffective orally. This study shows that spray drying is a suitable method for producing nanoparticulate vaccine formulations in dry powder form.

U2 - 10.1016/j.ijpharm.2018.08.036

DO - 10.1016/j.ijpharm.2018.08.036

M3 - Journal article

C2 - 30134183

SN - 0378-5173

VL - 550

SP - 35

EP - 44

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 1-2

ER -

Spray dried cubosomes with ovalbumin and Quil-A as a nanoparticulate dry powder vaccine formulation

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this