Splenocytes cultured in low concentrations of IL-2 generate NK cell specificities toward syngenic and allogenic targets.

TY - JOUR

T1 - Splenocytes cultured in low concentrations of IL-2 generate NK cell specificities toward syngenic and allogenic targets.

AU - Nissen, Mogens Holst

AU - Jeppesen, M

AU - Claesson, M H

N1 - Keywords: Animals; Cell Count; Cells, Cultured; Cytotoxicity, Immunologic; H-2 Antigens; Interleukin-2; Isoantigens; Killer Cells, Natural; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Nude; Phenotype; Spleen

PY - 2000

Y1 - 2000

N2 - Splenocytes cultured in the presence of 30-60 units/ml IL-2 for 5 days develop natural killer activity toward syngeneic and allogeneic tumor cell targets. The IL-2 activated splenocytes, themselves, are partially resistant, whereas concanavalin A-activated T blast cells are completely resistant to killing. Surprisingly, major histocompatibility complex (MHC)-I-negative target cells are also resistant to natural killer (NK)-cell-mediated killing. Cells resistant to killing were unable to block NK-cell-mediated killing of sensitive targets as judged from cold target cell inhibition experiments, and one type of target cells sensitive to killing did generally not cross-block killing of other killing-sensitive target cell types. Alloantigen exposure of splenocytes, i.e., one-way mixed lymphocyte cultures, partially prevents the development of NK-cell activity. Our data suggest that target structures which trigger killing activity of NK cells are determined by the phenotype of the target cell and are dependent on its MHC class I expression disregarding the haplotype of the cell.

AB - Splenocytes cultured in the presence of 30-60 units/ml IL-2 for 5 days develop natural killer activity toward syngeneic and allogeneic tumor cell targets. The IL-2 activated splenocytes, themselves, are partially resistant, whereas concanavalin A-activated T blast cells are completely resistant to killing. Surprisingly, major histocompatibility complex (MHC)-I-negative target cells are also resistant to natural killer (NK)-cell-mediated killing. Cells resistant to killing were unable to block NK-cell-mediated killing of sensitive targets as judged from cold target cell inhibition experiments, and one type of target cells sensitive to killing did generally not cross-block killing of other killing-sensitive target cell types. Alloantigen exposure of splenocytes, i.e., one-way mixed lymphocyte cultures, partially prevents the development of NK-cell activity. Our data suggest that target structures which trigger killing activity of NK cells are determined by the phenotype of the target cell and are dependent on its MHC class I expression disregarding the haplotype of the cell.

U2 - 10.1006/cimm.2000.1670

DO - 10.1006/cimm.2000.1670

M3 - Journal article

C2 - 10915561

SN - 0008-8749

VL - 203

SP - 47

EP - 54

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -