Specific T-cell recognition of the merozoite proteins rhoptry-associated protein 1 and erythrocyte-binding antigen 1 of Plasmodium falciparum

TY - JOUR

T1 - Specific T-cell recognition of the merozoite proteins rhoptry-associated protein 1 and erythrocyte-binding antigen 1 of Plasmodium falciparum

AU - Jakobsen, P H

AU - Hviid, L

AU - Theander, T G

AU - Afare, E A

AU - Ridley, R G

AU - Heegaard, P M

AU - Stuber, D

AU - Dalsgaard, K

AU - Nkrumah, F K

N1 - Keywords: Adolescent; Adult; Amino Acid Sequence; Antigens, Protozoan; Antigens, Surface; Carrier Proteins; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Humans; Immunity, Cellular; Immunoglobulin G; Immunoglobulin M; Lymphocyte Activation; Malaria, Falciparum; Merozoite Surface Protein 1; Middle Aged; Molecular Sequence Data; Protein Precursors; Protozoan Proteins; Receptors, Cell Surface; T-Lymphocytes

PY - 1993

Y1 - 1993

N2 - The merozoite proteins merozoite surface protein 1 (MSP-1) and rhoptry-associated protein 1 (RAP-1) and synthetic peptides containing sequences of MSP-1, RAP-1, and erythrocyte-binding antigen 1, induced in vitro proliferative responses of lymphocytes collected from Ghanaian blood donors living in an area with a high rate of transmission of malaria. Lymphocytes from a large proportion of the Ghanaian blood donors proliferated in response to the RAP-1 peptide, unlike those of Danish control blood donors, indicating that this sequence contains a malaria-specific T-cell epitope broadly recognized by individuals living in an area with a high transmission rate of malaria. Most of the donor plasma samples tested contained immunoglobulin G (IgG) and IgM antibodies recognizing the merozoite proteins, while only a minority showed high IgG reactivity to the synthetic peptides.

AB - The merozoite proteins merozoite surface protein 1 (MSP-1) and rhoptry-associated protein 1 (RAP-1) and synthetic peptides containing sequences of MSP-1, RAP-1, and erythrocyte-binding antigen 1, induced in vitro proliferative responses of lymphocytes collected from Ghanaian blood donors living in an area with a high rate of transmission of malaria. Lymphocytes from a large proportion of the Ghanaian blood donors proliferated in response to the RAP-1 peptide, unlike those of Danish control blood donors, indicating that this sequence contains a malaria-specific T-cell epitope broadly recognized by individuals living in an area with a high transmission rate of malaria. Most of the donor plasma samples tested contained immunoglobulin G (IgG) and IgM antibodies recognizing the merozoite proteins, while only a minority showed high IgG reactivity to the synthetic peptides.

M3 - Journal article

C2 - 8418048

SN - 0019-9567

VL - 61

SP - 268

EP - 273

JO - Infection and Immunity

JF - Infection and Immunity

IS - 1

ER -