Specific microRNAs Regulate Heat Stress Responses in Caenorhabditis elegans

Camilla Nehammer; Agnieszka Podolska; Sebastian D Mackowiak; Konstantinos Kagias; Roger David John Pocock

doi:10.1038/srep08866

Specific microRNAs Regulate Heat Stress Responses in Caenorhabditis elegans

Camilla Nehammer, Agnieszka Podolska, Sebastian D Mackowiak, Konstantinos Kagias, Roger David John Pocock

32 Citations (Scopus)

Abstract

The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

Original language	English
Journal	Scientific Reports
Volume	5
Pages (from-to)	8866
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep08866
Publication status	Published - 9 Mar 2015

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title = "Specific microRNAs Regulate Heat Stress Responses in Caenorhabditis elegans",

abstract = "The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.",

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T1 - Specific microRNAs Regulate Heat Stress Responses in Caenorhabditis elegans

AU - Nehammer, Camilla

AU - Podolska, Agnieszka

AU - Mackowiak, Sebastian D

AU - Kagias, Konstantinos

AU - Pocock, Roger David John

PY - 2015/3/9

Y1 - 2015/3/9

N2 - The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

AB - The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

U2 - 10.1038/srep08866

DO - 10.1038/srep08866

M3 - Journal article

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SN - 2045-2322

VL - 5

SP - 8866

JO - Scientific Reports

JF - Scientific Reports

ER -

Specific microRNAs Regulate Heat Stress Responses in Caenorhabditis elegans

Abstract

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