Some bacterial parameters influencing the neutrophil oxidative burst response to Pseudomonas aeruginosa biofilms

TY - JOUR

T1 - Some bacterial parameters influencing the neutrophil oxidative burst response to Pseudomonas aeruginosa biofilms

AU - Jensen, E T

AU - Kharazmi, A

AU - Høiby, N

AU - Costerton, J W

N1 - Keywords: Cystic Fibrosis; Humans; Luminescent Measurements; Neutrophils; Opsonin Proteins; Pseudomonas aeruginosa; Respiratory Burst; Superoxides

PY - 1992

Y1 - 1992

N2 - Persistence of bacteria in spite of a normal host immune system and relevant antibiotic treatment is a key problem in many chronic infections, such as the bronchopulmonary P. aeruginosa infection in cystic fibrosis patients. The capability of bacteria to establish themselves in microcolonies or biofilms is an important protective mechanism of the microorganisms. We examined the human PMN oxidative burst response to P. aeruginosa in biofilm and in planktonic form. The PMN chemiluminescence response to P. aeruginosa in biofilms was reduced to 30.5-47.5% (p less than 0.04) and the superoxide response to 85.9% (p less than 0.02) of the response to equivalent numbers of planktonic bacteria. Mechanical disruption of the biofilms before the assays elicited a significantly increased response in the chemiluminescence experiments and to nonopsonized biofilms in the superoxide anion experiments. We conclude that biofilm bacteria, although able to stimulate the PMN, result in a reduced, suboptimal response leading to lack of efficient eradication of the bacteria in the chronic infection.

AB - Persistence of bacteria in spite of a normal host immune system and relevant antibiotic treatment is a key problem in many chronic infections, such as the bronchopulmonary P. aeruginosa infection in cystic fibrosis patients. The capability of bacteria to establish themselves in microcolonies or biofilms is an important protective mechanism of the microorganisms. We examined the human PMN oxidative burst response to P. aeruginosa in biofilm and in planktonic form. The PMN chemiluminescence response to P. aeruginosa in biofilms was reduced to 30.5-47.5% (p less than 0.04) and the superoxide response to 85.9% (p less than 0.02) of the response to equivalent numbers of planktonic bacteria. Mechanical disruption of the biofilms before the assays elicited a significantly increased response in the chemiluminescence experiments and to nonopsonized biofilms in the superoxide anion experiments. We conclude that biofilm bacteria, although able to stimulate the PMN, result in a reduced, suboptimal response leading to lack of efficient eradication of the bacteria in the chronic infection.

M3 - Journal article

C2 - 1325804

SN - 0903-465X

VL - 100

SP - 727

EP - 733

JO - APMIS. Supplementum

JF - APMIS. Supplementum

IS - 8

ER -