Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity

P H Jakobsen; T G Theander; J B Jensen; K Mølbak; S Jepsen

Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity

P H Jakobsen, T G Theander, J B Jensen, K Mølbak, S Jepsen

Department of Immunology and Microbiology

18 Citations (Scopus)

Abstract

The importance of carbohydrate moieties for the antigenicity of purified soluble Plasmodium falciparum antigens from the asexual blood stage was tested. Digestion of the soluble antigens with alpha-D-galactosidase clearly affected the ability of the antigen to react with malaria-immune sera from different geographical origins in crossed immunoelectrophoresis and immunoblotting. Antigens of 220, 180, 80, and 74 kilodaltons were affected by the enzyme treatment. Furthermore, the enzyme digestion reduced the ability of the purified soluble antigen to stimulate lymphocytes from malaria-immune donors. The results might have important implications for the strategy of developing a malaria vaccine.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	25
Issue number	11
Pages (from-to)	2075-9
Number of pages	4
ISSN	0095-1137
Publication status	Published - 1987

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title = "Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity",

abstract = "The importance of carbohydrate moieties for the antigenicity of purified soluble Plasmodium falciparum antigens from the asexual blood stage was tested. Digestion of the soluble antigens with alpha-D-galactosidase clearly affected the ability of the antigen to react with malaria-immune sera from different geographical origins in crossed immunoelectrophoresis and immunoblotting. Antigens of 220, 180, 80, and 74 kilodaltons were affected by the enzyme treatment. Furthermore, the enzyme digestion reduced the ability of the purified soluble antigen to stimulate lymphocytes from malaria-immune donors. The results might have important implications for the strategy of developing a malaria vaccine.",

author = "Jakobsen, {P H} and Theander, {T G} and Jensen, {J B} and K M{\o}lbak and S Jepsen",

note = "Keywords: Animals; Antigens, Protozoan; Carbohydrates; Humans; Immunoassay; Immunoelectrophoresis, Two-Dimensional; Leukocytes, Mononuclear; Malaria; Plasmodium falciparum; alpha-Galactosidase",

year = "1987",

language = "English",

volume = "25",

pages = "2075--9",

journal = "Journal of Clinical Microbiology",

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T1 - Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity

AU - Jakobsen, P H

AU - Theander, T G

AU - Jensen, J B

AU - Mølbak, K

AU - Jepsen, S

N1 - Keywords: Animals; Antigens, Protozoan; Carbohydrates; Humans; Immunoassay; Immunoelectrophoresis, Two-Dimensional; Leukocytes, Mononuclear; Malaria; Plasmodium falciparum; alpha-Galactosidase

PY - 1987

Y1 - 1987

N2 - The importance of carbohydrate moieties for the antigenicity of purified soluble Plasmodium falciparum antigens from the asexual blood stage was tested. Digestion of the soluble antigens with alpha-D-galactosidase clearly affected the ability of the antigen to react with malaria-immune sera from different geographical origins in crossed immunoelectrophoresis and immunoblotting. Antigens of 220, 180, 80, and 74 kilodaltons were affected by the enzyme treatment. Furthermore, the enzyme digestion reduced the ability of the purified soluble antigen to stimulate lymphocytes from malaria-immune donors. The results might have important implications for the strategy of developing a malaria vaccine.

AB - The importance of carbohydrate moieties for the antigenicity of purified soluble Plasmodium falciparum antigens from the asexual blood stage was tested. Digestion of the soluble antigens with alpha-D-galactosidase clearly affected the ability of the antigen to react with malaria-immune sera from different geographical origins in crossed immunoelectrophoresis and immunoblotting. Antigens of 220, 180, 80, and 74 kilodaltons were affected by the enzyme treatment. Furthermore, the enzyme digestion reduced the ability of the purified soluble antigen to stimulate lymphocytes from malaria-immune donors. The results might have important implications for the strategy of developing a malaria vaccine.

M3 - Journal article

C2 - 2826533

SN - 0095-1137

VL - 25

SP - 2075

EP - 2079

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

Soluble Plasmodium falciparum antigens contain carbohydrate moieties important for immune reactivity

Abstract

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