Soil microbial toxicity of eight polycyclic aromatic compounds: effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans.

Line Emilie Sverdrup; Flemming Ekelund; Paul Henning Krogh; Torben Nielsen; Kaare Johnsen

doi:10.1897/1551-5028(2002)021<1644:SMTOEP>2.0.CO;2

Soil microbial toxicity of eight polycyclic aromatic compounds: effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans.

Line Emilie Sverdrup, Flemming Ekelund, Paul Henning Krogh, Torben Nielsen, Kaare Johnsen

Terrestrial Ecology

51 Citations (Scopus)

Abstract

Eight polycyclic aromatic compounds (PACs) were tested for their toxic effect on the soil nitrification process, bacterial genetic diversity, and the total number of protozoans (naked amoebae and heterotrophic flagellates). After four weeks of exposure in a well-characterized agricultural soil, toxic effects were evaluated by comparison to uncontaminated control soils. All PACs affected the nitrification process, and the calculated no-observed-effect concentrations (NOECs) for nitrification were 79 mg/kg for pyrene, 24 mg/kg for fluoranthene, 26 mg/kg for phenanthrene, 72 mg/kg for fluorene, 23 mg/kg for carbazole, 22 mg/kg for dibenzothiophene, 75 mg/kg for dibenzofuran, and 1,100 mg/kg for acridine. For all substances but acridine, nitrification was the most sensitive of the three toxicity indicators evaluated. No effect of the tested substances on bacterial diversity was found, as measured by denaturant gradient gel electrophoresis. In general, only weak effects at very high concentrations were found for the protozoans. However, for acridine, protozoan numbers were reduced at lower concentrations than those that affected the nitrification process, that is, with a 5% reduction at 380 mg/kg. For effects on nitrification, toxicity (NOEC values) expressed as soil pore-water concentrations (log10(micromol/L)) showed a significant inverse relationship with lipophilicity (log octanol-water partition coefficient) of the substances (r2 = 0.69, p = 0.011, n = 8). This finding could indicate that the toxicity of substances similar to those tested might be predicted by a quantitative structure-activity relationship with lipophilicity as the predictor variable.

Original language	English
Journal	Environmental Toxicology and Chemistry
Volume	21
Issue number	8
Pages (from-to)	1644-50
Number of pages	6
ISSN	0730-7268
DOIs	https://doi.org/10.1897/1551-5028(2002)021<1644:SMTOEP>2.0.CO;2
Publication status	Published - 2002

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Soil microbial toxicity of eight polycyclic aromatic compounds: effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans. / Sverdrup, Line Emilie; Ekelund, Flemming; Krogh, Paul Henning et al.

In: Environmental Toxicology and Chemistry, Vol. 21, No. 8, 2002, p. 1644-50.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{b1eda7e0b4b711ddb04f000ea68e967b,

title = "Soil microbial toxicity of eight polycyclic aromatic compounds: effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans.",

abstract = "Eight polycyclic aromatic compounds (PACs) were tested for their toxic effect on the soil nitrification process, bacterial genetic diversity, and the total number of protozoans (naked amoebae and heterotrophic flagellates). After four weeks of exposure in a well-characterized agricultural soil, toxic effects were evaluated by comparison to uncontaminated control soils. All PACs affected the nitrification process, and the calculated no-observed-effect concentrations (NOECs) for nitrification were 79 mg/kg for pyrene, 24 mg/kg for fluoranthene, 26 mg/kg for phenanthrene, 72 mg/kg for fluorene, 23 mg/kg for carbazole, 22 mg/kg for dibenzothiophene, 75 mg/kg for dibenzofuran, and 1,100 mg/kg for acridine. For all substances but acridine, nitrification was the most sensitive of the three toxicity indicators evaluated. No effect of the tested substances on bacterial diversity was found, as measured by denaturant gradient gel electrophoresis. In general, only weak effects at very high concentrations were found for the protozoans. However, for acridine, protozoan numbers were reduced at lower concentrations than those that affected the nitrification process, that is, with a 5% reduction at 380 mg/kg. For effects on nitrification, toxicity (NOEC values) expressed as soil pore-water concentrations (log10(micromol/L)) showed a significant inverse relationship with lipophilicity (log octanol-water partition coefficient) of the substances (r2 = 0.69, p = 0.011, n = 8). This finding could indicate that the toxicity of substances similar to those tested might be predicted by a quantitative structure-activity relationship with lipophilicity as the predictor variable.",

author = "Sverdrup, {Line Emilie} and Flemming Ekelund and Krogh, {Paul Henning} and Torben Nielsen and Kaare Johnsen",

note = "Keywords: Animals; Bacteria; Genetics, Population; Lipids; Nitrogen; No-Observed-Adverse-Effect Level; Polycyclic Hydrocarbons, Aromatic; Population Dynamics; Protozoa; Soil; Soil Microbiology; Soil Pollutants; Solubility; Structure-Activity Relationship",

year = "2002",

doi = "10.1897/1551-5028(2002)021<1644:SMTOEP>2.0.CO;2",

language = "English",

volume = "21",

pages = "1644--50",

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T1 - Soil microbial toxicity of eight polycyclic aromatic compounds: effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans.

AU - Sverdrup, Line Emilie

AU - Ekelund, Flemming

AU - Krogh, Paul Henning

AU - Nielsen, Torben

AU - Johnsen, Kaare

N1 - Keywords: Animals; Bacteria; Genetics, Population; Lipids; Nitrogen; No-Observed-Adverse-Effect Level; Polycyclic Hydrocarbons, Aromatic; Population Dynamics; Protozoa; Soil; Soil Microbiology; Soil Pollutants; Solubility; Structure-Activity Relationship

PY - 2002

Y1 - 2002

N2 - Eight polycyclic aromatic compounds (PACs) were tested for their toxic effect on the soil nitrification process, bacterial genetic diversity, and the total number of protozoans (naked amoebae and heterotrophic flagellates). After four weeks of exposure in a well-characterized agricultural soil, toxic effects were evaluated by comparison to uncontaminated control soils. All PACs affected the nitrification process, and the calculated no-observed-effect concentrations (NOECs) for nitrification were 79 mg/kg for pyrene, 24 mg/kg for fluoranthene, 26 mg/kg for phenanthrene, 72 mg/kg for fluorene, 23 mg/kg for carbazole, 22 mg/kg for dibenzothiophene, 75 mg/kg for dibenzofuran, and 1,100 mg/kg for acridine. For all substances but acridine, nitrification was the most sensitive of the three toxicity indicators evaluated. No effect of the tested substances on bacterial diversity was found, as measured by denaturant gradient gel electrophoresis. In general, only weak effects at very high concentrations were found for the protozoans. However, for acridine, protozoan numbers were reduced at lower concentrations than those that affected the nitrification process, that is, with a 5% reduction at 380 mg/kg. For effects on nitrification, toxicity (NOEC values) expressed as soil pore-water concentrations (log10(micromol/L)) showed a significant inverse relationship with lipophilicity (log octanol-water partition coefficient) of the substances (r2 = 0.69, p = 0.011, n = 8). This finding could indicate that the toxicity of substances similar to those tested might be predicted by a quantitative structure-activity relationship with lipophilicity as the predictor variable.

AB - Eight polycyclic aromatic compounds (PACs) were tested for their toxic effect on the soil nitrification process, bacterial genetic diversity, and the total number of protozoans (naked amoebae and heterotrophic flagellates). After four weeks of exposure in a well-characterized agricultural soil, toxic effects were evaluated by comparison to uncontaminated control soils. All PACs affected the nitrification process, and the calculated no-observed-effect concentrations (NOECs) for nitrification were 79 mg/kg for pyrene, 24 mg/kg for fluoranthene, 26 mg/kg for phenanthrene, 72 mg/kg for fluorene, 23 mg/kg for carbazole, 22 mg/kg for dibenzothiophene, 75 mg/kg for dibenzofuran, and 1,100 mg/kg for acridine. For all substances but acridine, nitrification was the most sensitive of the three toxicity indicators evaluated. No effect of the tested substances on bacterial diversity was found, as measured by denaturant gradient gel electrophoresis. In general, only weak effects at very high concentrations were found for the protozoans. However, for acridine, protozoan numbers were reduced at lower concentrations than those that affected the nitrification process, that is, with a 5% reduction at 380 mg/kg. For effects on nitrification, toxicity (NOEC values) expressed as soil pore-water concentrations (log10(micromol/L)) showed a significant inverse relationship with lipophilicity (log octanol-water partition coefficient) of the substances (r2 = 0.69, p = 0.011, n = 8). This finding could indicate that the toxicity of substances similar to those tested might be predicted by a quantitative structure-activity relationship with lipophilicity as the predictor variable.

U2 - 10.1897/1551-5028(2002)021<1644:SMTOEP>2.0.CO;2

DO - 10.1897/1551-5028(2002)021<1644:SMTOEP>2.0.CO;2

M3 - Journal article

C2 - 12152764

SN - 0730-7268

VL - 21

SP - 1644

EP - 1650

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

IS - 8

ER -

Soil microbial toxicity of eight polycyclic aromatic compounds: effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans.

Abstract

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