SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function

TY - JOUR

T1 - SOCS5 is expressed in primary B and T lymphoid cells but is dispensable for lymphocyte production and function

AU - Brender, Christine

AU - Columbus, Ruth

AU - Metcalf, Donald

AU - Handman, Emanuela

AU - Starr, Robyn

AU - Huntington, Nick

AU - Tarlinton, David

AU - Ødum, Niels

AU - Nicholson, Sandra E

AU - Nicola, Nicos A

AU - Hilton, Douglas J

AU - Alexander, Warren S

N1 - Keywords: Animals; B-Lymphocytes; Cell Differentiation; Female; Hematopoiesis; Leishmania major; Leishmaniasis, Cutaneous; Lymphocyte Activation; Mice; Mice, Knockout; Protein Biosynthesis; Proteins; Suppressor of Cytokine Signaling Proteins; T-Lymphocytes; T-Lymphocytes, Helper-Inducer

PY - 2004

Y1 - 2004

N2 - Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell responses, whereas the roles of other SOCS family members in the regulation of lymphocyte function are less clear. Here, we report the generation of mice with a targeted disruption of the Socs5 gene. Socs5(-/-) mice were born in a normal Mendelian ratio and were healthy and fertile. We found that SOCS5 is expressed in primary B and T cells in wild-type mice. However, no abnormalities in the lymphocyte compartment were seen in SOCS5-deficient mice. We examined antigen- and cytokine-induced proliferative responses in B and T cells in the absence of SOCS5 and found no deviations from the responses seen in wild-type cells. Because SOCS5 has been implicated in Th1 differentiation, we also investigated the importance of SOCS5 in T helper cell responses. Unexpectedly, SOCS5-deficient CD4 T cells showed no abnormalities in Th1/Th2 differentiation and Socs5(-/-) mice showed normal resistance to infection with Leishmania major. Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function.

AB - Suppressors of cytokine signaling (SOCSs) are key regulators of cytokine-induced responses in hematopoietic as well as nonhematopoietic cells. SOCS1 and SOCS3 have been shown to modulate T-cell responses, whereas the roles of other SOCS family members in the regulation of lymphocyte function are less clear. Here, we report the generation of mice with a targeted disruption of the Socs5 gene. Socs5(-/-) mice were born in a normal Mendelian ratio and were healthy and fertile. We found that SOCS5 is expressed in primary B and T cells in wild-type mice. However, no abnormalities in the lymphocyte compartment were seen in SOCS5-deficient mice. We examined antigen- and cytokine-induced proliferative responses in B and T cells in the absence of SOCS5 and found no deviations from the responses seen in wild-type cells. Because SOCS5 has been implicated in Th1 differentiation, we also investigated the importance of SOCS5 in T helper cell responses. Unexpectedly, SOCS5-deficient CD4 T cells showed no abnormalities in Th1/Th2 differentiation and Socs5(-/-) mice showed normal resistance to infection with Leishmania major. Therefore, although SOCS5 is expressed in primary B and T cells, it appears to be dispensable for the regulation of lymphocyte function.

U2 - 10.1128/MCB.24.13.6094-6103.2004

DO - 10.1128/MCB.24.13.6094-6103.2004

M3 - Journal article

C2 - 15199163

SN - 0270-7306

VL - 24

SP - 6094

EP - 6103

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 13

ER -