Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match

Joachim Nielsen; Peter Krustrup; Lars Nybo; Thomas G P Gunnarsson; Klavs Madsen; Henrik Daa Schrøder; Jens Bangsbo; Niels Ørtenblad

doi:10.1007/s00421-012-2341-9

Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match

Joachim Nielsen, Peter Krustrup, Lars Nybo, Thomas G P Gunnarsson, Klavs Madsen, Henrik Daa Schrøder, Jens Bangsbo, Niels Ørtenblad

19 Citations (Scopus)

Abstract

Whole muscle glycogen levels remain low for a prolonged period following a soccer match. The present study was conducted to investigate how this relates to glycogen content and particle size in distinct subcellular localizations. Seven high-level male soccer players had a vastus lateralis muscle biopsy collected immediately after and 24, 48, 72 and 120 h after a competitive soccer match. Transmission electron microscopy was used to estimate the subcellular distribution of glycogen and individual particle size. During the first day of recovery, glycogen content increased by ~60% in all subcellular localizations, but during the subsequent second day of recovery glycogen content located within the myofibrils (Intramyofibrillar glycogen, a minor deposition constituting 10-15% of total glycogen) did not increase further compared with an increase in subsarcolemmal glycogen (-7 vs. +25%, respectively, P = 0.047). Conversely, from the second to the fifth day of recovery, glycogen content increased (53%) within the myofibrils compared to no change in subsarcolemmal or intermyofibrillar glycogen (P

Original language	English
Journal	European Journal of Applied Physiology
Volume	112
Issue number	10
Pages (from-to)	3559-3567
Number of pages	9
ISSN	1439-6319
DOIs	https://doi.org/10.1007/s00421-012-2341-9
Publication status	Published - Oct 2012

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Nielsen, J., Krustrup, P., Nybo, L., Gunnarsson, T. G. P., Madsen, K., Schrøder, H. D., Bangsbo, J., & Ørtenblad, N. (2012). Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match. European Journal of Applied Physiology, 112(10), 3559-3567. https://doi.org/10.1007/s00421-012-2341-9

Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match. / Nielsen, Joachim; Krustrup, Peter; Nybo, Lars et al.

In: European Journal of Applied Physiology, Vol. 112, No. 10, 10.2012, p. 3559-3567.

Research output: Contribution to journal › Journal article › Research › peer-review

Nielsen, J, Krustrup, P, Nybo, L, Gunnarsson, TGP, Madsen, K, Schrøder, HD, Bangsbo, J & Ørtenblad, N 2012, 'Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match', European Journal of Applied Physiology, vol. 112, no. 10, pp. 3559-3567. https://doi.org/10.1007/s00421-012-2341-9

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abstract = "Whole muscle glycogen levels remain low for a prolonged period following a soccer match. The present study was conducted to investigate how this relates to glycogen content and particle size in distinct subcellular localizations. Seven high-level male soccer players had a vastus lateralis muscle biopsy collected immediately after and 24, 48, 72 and 120 h after a competitive soccer match. Transmission electron microscopy was used to estimate the subcellular distribution of glycogen and individual particle size. During the first day of recovery, glycogen content increased by ~60% in all subcellular localizations, but during the subsequent second day of recovery glycogen content located within the myofibrils (Intramyofibrillar glycogen, a minor deposition constituting 10-15% of total glycogen) did not increase further compared with an increase in subsarcolemmal glycogen (-7 vs. +25%, respectively, P = 0.047). Conversely, from the second to the fifth day of recovery, glycogen content increased (53%) within the myofibrils compared to no change in subsarcolemmal or intermyofibrillar glycogen (P ",

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AB - Whole muscle glycogen levels remain low for a prolonged period following a soccer match. The present study was conducted to investigate how this relates to glycogen content and particle size in distinct subcellular localizations. Seven high-level male soccer players had a vastus lateralis muscle biopsy collected immediately after and 24, 48, 72 and 120 h after a competitive soccer match. Transmission electron microscopy was used to estimate the subcellular distribution of glycogen and individual particle size. During the first day of recovery, glycogen content increased by ~60% in all subcellular localizations, but during the subsequent second day of recovery glycogen content located within the myofibrils (Intramyofibrillar glycogen, a minor deposition constituting 10-15% of total glycogen) did not increase further compared with an increase in subsarcolemmal glycogen (-7 vs. +25%, respectively, P = 0.047). Conversely, from the second to the fifth day of recovery, glycogen content increased (53%) within the myofibrils compared to no change in subsarcolemmal or intermyofibrillar glycogen (P

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Skeletal muscle glycogen content and particle size of distinct subcellular localizations in the recovery period after a high-level soccer match

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