Single framework recombinant antibody fragments designed for protein chip applications.

Cornelia Steinhauer; Christer Wingren; Ann-Christin Malmborg Hager; Carl A K Borrebaeck

Single framework recombinant antibody fragments designed for protein chip applications.

Cornelia Steinhauer, Christer Wingren, Ann-Christin Malmborg Hager, Carl A K Borrebaeck

66 Citations (Scopus)

Abstract

High-throughput proteomics, based on the microarray platform, requires stable, highly functional components that will yield a highly sensitive read-out of low abundance proteins. Although antibodies are the best characterized binding molecules for this purpose, only a fraction of them appear to behave satisfactorily in the chip format. Therefore, high demands need to be placed on their molecular design. In the present study, we have focused on recombinant antibody design based on a single framework for protein chip applications, aiming at defining crucial molecular probe parameters. Our results show that engineered human recombinant scFv antibody fragments that displayed appropriate biophysical properties (molecular [functional] stability in particular) can be generated, making them prime candidates for high-density antibody arrays. In fact, a superior framework that displays both multifaceted adsorption properties and very high functional stability over several months on chips (stored in a dried-out state) was identified. Taken together, designed scFv fragments based on a single molecular scaffold, readily accessible in large phage display libraries, can undoubtedly meet the requirements of probe content in antibody microarrays, particularly for global proteome analysis.

Original language	English
Journal	BioTechniques
Volume	Suppl
Pages (from-to)	38-45
Number of pages	7
ISSN	0736-6205
Publication status	Published - 2002
Externally published	Yes

Cite this

@article{a734ba0057c411dd8d9f000ea68e967b,

title = "Single framework recombinant antibody fragments designed for protein chip applications.",

abstract = "High-throughput proteomics, based on the microarray platform, requires stable, highly functional components that will yield a highly sensitive read-out of low abundance proteins. Although antibodies are the best characterized binding molecules for this purpose, only a fraction of them appear to behave satisfactorily in the chip format. Therefore, high demands need to be placed on their molecular design. In the present study, we have focused on recombinant antibody design based on a single framework for protein chip applications, aiming at defining crucial molecular probe parameters. Our results show that engineered human recombinant scFv antibody fragments that displayed appropriate biophysical properties (molecular [functional] stability in particular) can be generated, making them prime candidates for high-density antibody arrays. In fact, a superior framework that displays both multifaceted adsorption properties and very high functional stability over several months on chips (stored in a dried-out state) was identified. Taken together, designed scFv fragments based on a single molecular scaffold, readily accessible in large phage display libraries, can undoubtedly meet the requirements of probe content in antibody microarrays, particularly for global proteome analysis.",

author = "Cornelia Steinhauer and Christer Wingren and Hager, {Ann-Christin Malmborg} and Borrebaeck, {Carl A K}",

note = "Keywords: Antibodies, Monoclonal; Equipment Design; Equipment Failure Analysis; Humans; Immunoglobulin Fragments; Peptide Library; Protein Array Analysis; Proteins; Proteomics; Recombinant Proteins; Sequence Analysis, Protein",

year = "2002",

language = "English",

volume = "Suppl",

pages = "38--45",

journal = "BioTechniques",

issn = "0736-6205",

publisher = "Informa Healthcare",

}

TY - JOUR

T1 - Single framework recombinant antibody fragments designed for protein chip applications.

AU - Steinhauer, Cornelia

AU - Wingren, Christer

AU - Hager, Ann-Christin Malmborg

AU - Borrebaeck, Carl A K

N1 - Keywords: Antibodies, Monoclonal; Equipment Design; Equipment Failure Analysis; Humans; Immunoglobulin Fragments; Peptide Library; Protein Array Analysis; Proteins; Proteomics; Recombinant Proteins; Sequence Analysis, Protein

PY - 2002

Y1 - 2002

N2 - High-throughput proteomics, based on the microarray platform, requires stable, highly functional components that will yield a highly sensitive read-out of low abundance proteins. Although antibodies are the best characterized binding molecules for this purpose, only a fraction of them appear to behave satisfactorily in the chip format. Therefore, high demands need to be placed on their molecular design. In the present study, we have focused on recombinant antibody design based on a single framework for protein chip applications, aiming at defining crucial molecular probe parameters. Our results show that engineered human recombinant scFv antibody fragments that displayed appropriate biophysical properties (molecular [functional] stability in particular) can be generated, making them prime candidates for high-density antibody arrays. In fact, a superior framework that displays both multifaceted adsorption properties and very high functional stability over several months on chips (stored in a dried-out state) was identified. Taken together, designed scFv fragments based on a single molecular scaffold, readily accessible in large phage display libraries, can undoubtedly meet the requirements of probe content in antibody microarrays, particularly for global proteome analysis.

AB - High-throughput proteomics, based on the microarray platform, requires stable, highly functional components that will yield a highly sensitive read-out of low abundance proteins. Although antibodies are the best characterized binding molecules for this purpose, only a fraction of them appear to behave satisfactorily in the chip format. Therefore, high demands need to be placed on their molecular design. In the present study, we have focused on recombinant antibody design based on a single framework for protein chip applications, aiming at defining crucial molecular probe parameters. Our results show that engineered human recombinant scFv antibody fragments that displayed appropriate biophysical properties (molecular [functional] stability in particular) can be generated, making them prime candidates for high-density antibody arrays. In fact, a superior framework that displays both multifaceted adsorption properties and very high functional stability over several months on chips (stored in a dried-out state) was identified. Taken together, designed scFv fragments based on a single molecular scaffold, readily accessible in large phage display libraries, can undoubtedly meet the requirements of probe content in antibody microarrays, particularly for global proteome analysis.

M3 - Journal article

C2 - 12514928

SN - 0736-6205

VL - Suppl

SP - 38

EP - 45

JO - BioTechniques

JF - BioTechniques

ER -

Single framework recombinant antibody fragments designed for protein chip applications.

Abstract

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