Single-cell Analysis of Lambda Immunity Regulation

Kristoffer Torbjørn Bæk; Sine Lo Svenningsen; Harvey Eisen; Kim Sneppen; Stanley Brown

doi:10.1016/j.jmb.2003.09.037

Single-cell Analysis of Lambda Immunity Regulation

Kristoffer Torbjørn Bæk, Sine Lo Svenningsen, Harvey Eisen, Kim Sneppen, Stanley Brown

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Abstract

We have examined expression of the ¿cI operon in single cells via a rexgfp substitution. Although average fluorescence agreed with expectations for expression of ¿-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.

Original language	English
Journal	Journal of Molecular Biology
Volume	334
Issue number	3
Pages (from-to)	363-372
Number of pages	10
ISSN	0022-2836
DOIs	https://doi.org/10.1016/j.jmb.2003.09.037
Publication status	Published - 2003

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single cell analysis of lambda immunity regulationSubmitted manuscript, 656 KB

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@article{2285ca3017bf11df8ed1000ea68e967b,

title = "Single-cell Analysis of Lambda Immunity Regulation",

abstract = "We have examined expression of the ¿cI operon in single cells via a rexgfp substitution. Although average fluorescence agreed with expectations for expression of ¿-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.",

author = "B{\ae}k, {Kristoffer Torbj{\o}rn} and Svenningsen, {Sine Lo} and Harvey Eisen and Kim Sneppen and Stanley Brown",

note = "Keywords: bacteriophage lambda; repressor; GFP; single-cell analyses",

year = "2003",

doi = "10.1016/j.jmb.2003.09.037",

language = "English",

volume = "334",

pages = "363--372",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

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T1 - Single-cell Analysis of Lambda Immunity Regulation

AU - Bæk, Kristoffer Torbjørn

AU - Svenningsen, Sine Lo

AU - Eisen, Harvey

AU - Sneppen, Kim

AU - Brown, Stanley

N1 - Keywords: bacteriophage lambda; repressor; GFP; single-cell analyses

PY - 2003

Y1 - 2003

N2 - We have examined expression of the ¿cI operon in single cells via a rexgfp substitution. Although average fluorescence agreed with expectations for expression of ¿-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.

AB - We have examined expression of the ¿cI operon in single cells via a rexgfp substitution. Although average fluorescence agreed with expectations for expression of ¿-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage.

U2 - 10.1016/j.jmb.2003.09.037

DO - 10.1016/j.jmb.2003.09.037

M3 - Journal article

SN - 0022-2836

VL - 334

SP - 363

EP - 372

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 3

ER -

Single-cell Analysis of Lambda Immunity Regulation

Abstract

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