TY - JOUR
T1 - Simultaneous extraction of acidic and basic drugs at neutral sample pH
T2 - a novel electro-mediated microextraction approach
AU - Basheer, Chanbasha
AU - Lee, Jingyi
AU - Pedersen-Bjergaard, Stig
AU - Rasmussen , Knut Einar
AU - Lee, Hian Kee
PY - 2010/10/22
Y1 - 2010/10/22
N2 - The simultaneous extraction of acidic and basic analytes from a particular sample is a challenging task. In this work, electromembrane extraction (EME) of acidic non-steroidal anti-inflammatory drugs and basic β-blockers in a single step was carried out for the first time. It was shown that by designing an appropriate compartmentalized membrane envelope, the two classes of drugs could be electrokinetically extracted by a 300V direct current electrical potential. This method required only a very short 10-min extraction time from a pH-neutral sample, with a small amount (50μL) of organic solvent (1-octanol) as the acceptor phase. Analysis was carried out using gas chromatography-mass spectrometry after derivatization of the analytes. Extraction parameters such as extraction time, applied voltage, pH range, and concentration of salt added were optimized. The proposed EME technique provided good linearity with correlation coefficients from 0.982 to 0.997 over a concentration range of 1-200μgL-1. Detection limits of the drugs ranged between 0.0081 and 0.26μgL-1, while reproducibility ranged from 6 to 13% (n=6). Finally, the application of the new method to wastewater samples was demonstrated.
AB - The simultaneous extraction of acidic and basic analytes from a particular sample is a challenging task. In this work, electromembrane extraction (EME) of acidic non-steroidal anti-inflammatory drugs and basic β-blockers in a single step was carried out for the first time. It was shown that by designing an appropriate compartmentalized membrane envelope, the two classes of drugs could be electrokinetically extracted by a 300V direct current electrical potential. This method required only a very short 10-min extraction time from a pH-neutral sample, with a small amount (50μL) of organic solvent (1-octanol) as the acceptor phase. Analysis was carried out using gas chromatography-mass spectrometry after derivatization of the analytes. Extraction parameters such as extraction time, applied voltage, pH range, and concentration of salt added were optimized. The proposed EME technique provided good linearity with correlation coefficients from 0.982 to 0.997 over a concentration range of 1-200μgL-1. Detection limits of the drugs ranged between 0.0081 and 0.26μgL-1, while reproducibility ranged from 6 to 13% (n=6). Finally, the application of the new method to wastewater samples was demonstrated.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1016/j.chroma.2010.04.066
DO - 10.1016/j.chroma.2010.04.066
M3 - Journal article
C2 - 20488447
SN - 0301-4770
VL - 1217
SP - 6661
EP - 6667
JO - Journal of Chromatography
JF - Journal of Chromatography
IS - 43
ER -