Abstract
Immunohistochemical study of the distribution of carbohydrate core-structures on O-linked glycoproteins (T, sialosyl-T, Tn and sialosyl-Tn) was performed using specific monoclonal antibodies on 148 primary breast lesions, including 10 normal breast tissues, 16 benign lesions and 122 invasive carcinomas (79 localized and 43 metastatic lesions). T antigen, not observed in normal breast tissue, was present in 31% of the benign lesions and in some cases of morphologically normal epithelium adjacent to tumor cells, compatible with altered glycosylation being an early event. Sialosyl-T (s-T) antigen was present in all cases of normal epithelium and in 81% of the benign lesions. Both Tn and sialosyl-Tn (s-Tn) antigen were present in normal breast lesions. Both Tn and sialosyl-Tn (s-Tn) antigen were present in normal breast tissue (30%) and benign lesions (31% and 19%). In the malignant lesions, 20% were positive for T antigen, 82% for s-T antigen, 66% for Tn antigen and 22% for s-Tn antigen. The staining pattern was nearly identical for carcinomas with and without lymph node metastases. In conclusion, immunostaining for simple mucins does not permit a clear distinction between benign and malignant breast lesions.
| Original language | English |
|---|---|
| Journal | Anticancer Research |
| Volume | 14 |
| Issue number | 2B |
| Pages (from-to) | 609-15 |
| Number of pages | 7 |
| ISSN | 0250-7005 |
| Publication status | Published - 1 Mar 1994 |
Keywords
- Antibodies, Monoclonal
- Antigens, Tumor-Associated, Carbohydrate
- Antigens, Viral, Tumor
- Biological Markers
- Breast
- Breast Neoplasms
- Carbohydrate Sequence
- Carbohydrates
- Carcinoma, Ductal, Breast
- Epithelial Cells
- Epithelium
- Epitopes
- Female
- Humans
- Immunohistochemistry
- Molecular Sequence Data
- Mucins
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Oligosaccharides
- Tumor Markers, Biological