Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants

Alexandra Jammer; Anna Gasperl; Nora Luschin-Ebengreuth; Elmien Heyneke; Hyosub Chu; Elena Cantero-Navarro; Dominik Kilian Grosskinsky; Alfonso A. Albacete; Edith Stabentheiner; Jürgen Franzaring; Andreas Fangmeier; Eric van der Graaff; Thomas Georg Roitsch

doi:10.1093/jxb/erv228

Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants

Alexandra Jammer, Anna Gasperl, Nora Luschin-Ebengreuth, Elmien Heyneke, Hyosub Chu, Elena Cantero-Navarro, Dominik Kilian Grosskinsky, Alfonso A. Albacete, Edith Stabentheiner, Jürgen Franzaring, Andreas Fangmeier, Eric van der Graaff, Thomas Georg Roitsch

Section for Crop Sciences

36 Citations (Scopus)

Abstract

The analysis of physiological parameters is important to understand the link between plant phenotypes and their genetic bases, and therefore is needed as an important element in the analysis of model and crop plants. The activities of enzymes involved in primary carbohydrate metabolism have been shown to be strongly associated with growth performance, crop yield, and quality, as well as stress responses. A simple, fast, and cost-effective method to determine activities for 13 key enzymes involved in carbohydrate metabolism has been established, mainly based on coupled spectrophotometric kinetic assays. The comparison of extraction buffers and requirement for dialysis of crude protein extracts resulted in a universal protein extraction protocol, suitable for the preparation of protein extracts from different organs of various species. Individual published kinetic activity assays were optimized and adapted for a semi-high-throughput 96-well assay format. These assays proved to be robust and are thus suitable for physiological phenotyping, enabling the characterization and diagnosis of the physiological state. The potential of the determination of distinct enzyme activity signatures as part of a physiological fingerprint was shown for various organs and tissues from three monocot and five dicot model and crop species, including two case studies with external stimuli. Differential and specific enzyme activity signatures are apparent during inflorescence development and upon in vitro cold treatment of young inflorescences in the monocot ryegrass, related to conditions for doubled haploid formation. Likewise, treatment of dicot spring oilseed rape with elevated CO2 concentration resulted in distinct patterns of enzyme activity responses in leaves.

Original language	English
Journal	Journal of Experimental Botany
Volume	66
Issue number	18
Pages (from-to)	5531-5542
Number of pages	12
ISSN	0022-0957
DOIs	https://doi.org/10.1093/jxb/erv228
Publication status	Published - 1 Sept 2015

Access to Document

10.1093/jxb/erv228

erv228.pdfFinal published version, 1.29 MB

Cite this

Jammer, A., Gasperl, A., Luschin-Ebengreuth, N., Heyneke, E., Chu, H., Cantero-Navarro, E., Grosskinsky, D. K., Albacete, A. A., Stabentheiner, E., Franzaring, J., Fangmeier, A., van der Graaff, E., & Roitsch, T. G. (2015). Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants. Journal of Experimental Botany, 66(18), 5531-5542. https://doi.org/10.1093/jxb/erv228

Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants. / Jammer, Alexandra; Gasperl, Anna; Luschin-Ebengreuth, Nora et al.

In: Journal of Experimental Botany, Vol. 66, No. 18, 01.09.2015, p. 5531-5542.

Research output: Contribution to journal › Journal article › Research › peer-review

Jammer, A, Gasperl, A, Luschin-Ebengreuth, N, Heyneke, E, Chu, H, Cantero-Navarro, E, Grosskinsky, DK, Albacete, AA, Stabentheiner, E, Franzaring, J, Fangmeier, A, van der Graaff, E & Roitsch, TG 2015, 'Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants', Journal of Experimental Botany, vol. 66, no. 18, pp. 5531-5542. https://doi.org/10.1093/jxb/erv228

@article{6963d3a4638249b5ba4fde9334c47a6b,

title = "Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants",

abstract = "The analysis of physiological parameters is important to understand the link between plant phenotypes and their genetic bases, and therefore is needed as an important element in the analysis of model and crop plants. The activities of enzymes involved in primary carbohydrate metabolism have been shown to be strongly associated with growth performance, crop yield, and quality, as well as stress responses. A simple, fast, and cost-effective method to determine activities for 13 key enzymes involved in carbohydrate metabolism has been established, mainly based on coupled spectrophotometric kinetic assays. The comparison of extraction buffers and requirement for dialysis of crude protein extracts resulted in a universal protein extraction protocol, suitable for the preparation of protein extracts from different organs of various species. Individual published kinetic activity assays were optimized and adapted for a semi-high-throughput 96-well assay format. These assays proved to be robust and are thus suitable for physiological phenotyping, enabling the characterization and diagnosis of the physiological state. The potential of the determination of distinct enzyme activity signatures as part of a physiological fingerprint was shown for various organs and tissues from three monocot and five dicot model and crop species, including two case studies with external stimuli. Differential and specific enzyme activity signatures are apparent during inflorescence development and upon in vitro cold treatment of young inflorescences in the monocot ryegrass, related to conditions for doubled haploid formation. Likewise, treatment of dicot spring oilseed rape with elevated CO2 concentration resulted in distinct patterns of enzyme activity responses in leaves.",

author = "Alexandra Jammer and Anna Gasperl and Nora Luschin-Ebengreuth and Elmien Heyneke and Hyosub Chu and Elena Cantero-Navarro and Grosskinsky, {Dominik Kilian} and Albacete, {Alfonso A.} and Edith Stabentheiner and J{\"u}rgen Franzaring and Andreas Fangmeier and {van der Graaff}, Eric and Roitsch, {Thomas Georg}",

year = "2015",

month = sep,

day = "1",

doi = "10.1093/jxb/erv228",

language = "English",

volume = "66",

pages = "5531--5542",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

publisher = "Oxford University Press",

number = "18",

}

TY - JOUR

T1 - Simple and robust determination of the activity signature of key carbohydrate metabolism enzymes for physiological phenotyping in model and crop plants

AU - Jammer, Alexandra

AU - Gasperl, Anna

AU - Luschin-Ebengreuth, Nora

AU - Heyneke, Elmien

AU - Chu, Hyosub

AU - Cantero-Navarro, Elena

AU - Grosskinsky, Dominik Kilian

AU - Albacete, Alfonso A.

AU - Stabentheiner, Edith

AU - Franzaring, Jürgen

AU - Fangmeier, Andreas

AU - van der Graaff, Eric

AU - Roitsch, Thomas Georg

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The analysis of physiological parameters is important to understand the link between plant phenotypes and their genetic bases, and therefore is needed as an important element in the analysis of model and crop plants. The activities of enzymes involved in primary carbohydrate metabolism have been shown to be strongly associated with growth performance, crop yield, and quality, as well as stress responses. A simple, fast, and cost-effective method to determine activities for 13 key enzymes involved in carbohydrate metabolism has been established, mainly based on coupled spectrophotometric kinetic assays. The comparison of extraction buffers and requirement for dialysis of crude protein extracts resulted in a universal protein extraction protocol, suitable for the preparation of protein extracts from different organs of various species. Individual published kinetic activity assays were optimized and adapted for a semi-high-throughput 96-well assay format. These assays proved to be robust and are thus suitable for physiological phenotyping, enabling the characterization and diagnosis of the physiological state. The potential of the determination of distinct enzyme activity signatures as part of a physiological fingerprint was shown for various organs and tissues from three monocot and five dicot model and crop species, including two case studies with external stimuli. Differential and specific enzyme activity signatures are apparent during inflorescence development and upon in vitro cold treatment of young inflorescences in the monocot ryegrass, related to conditions for doubled haploid formation. Likewise, treatment of dicot spring oilseed rape with elevated CO2 concentration resulted in distinct patterns of enzyme activity responses in leaves.

AB - The analysis of physiological parameters is important to understand the link between plant phenotypes and their genetic bases, and therefore is needed as an important element in the analysis of model and crop plants. The activities of enzymes involved in primary carbohydrate metabolism have been shown to be strongly associated with growth performance, crop yield, and quality, as well as stress responses. A simple, fast, and cost-effective method to determine activities for 13 key enzymes involved in carbohydrate metabolism has been established, mainly based on coupled spectrophotometric kinetic assays. The comparison of extraction buffers and requirement for dialysis of crude protein extracts resulted in a universal protein extraction protocol, suitable for the preparation of protein extracts from different organs of various species. Individual published kinetic activity assays were optimized and adapted for a semi-high-throughput 96-well assay format. These assays proved to be robust and are thus suitable for physiological phenotyping, enabling the characterization and diagnosis of the physiological state. The potential of the determination of distinct enzyme activity signatures as part of a physiological fingerprint was shown for various organs and tissues from three monocot and five dicot model and crop species, including two case studies with external stimuli. Differential and specific enzyme activity signatures are apparent during inflorescence development and upon in vitro cold treatment of young inflorescences in the monocot ryegrass, related to conditions for doubled haploid formation. Likewise, treatment of dicot spring oilseed rape with elevated CO2 concentration resulted in distinct patterns of enzyme activity responses in leaves.

U2 - 10.1093/jxb/erv228

DO - 10.1093/jxb/erv228

M3 - Journal article

C2 - 26002973

SN - 0022-0957

VL - 66

SP - 5531

EP - 5542

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 18

ER -