Silver against Pseudomonas aeruginosa biofilms

Thomas Bjarnsholt; Klaus Kirketerp-Møller; Søren Kristiansen; Richard Phipps; Anne Kirstine Nielsen; Peter Østrup Jensen; Niels Høiby; Michael Givskov

doi:10.1111/j.1600-0463.2007.apm_646.x

Silver against Pseudomonas aeruginosa biofilms

Thomas Bjarnsholt, Klaus Kirketerp-Møller, Søren Kristiansen, Richard Phipps, Anne Kirstine Nielsen, Peter Østrup Jensen, Niels Høiby, Michael Givskov

Department of Immunology and Microbiology

146 Citations (Scopus)

Abstract

Silver has been recognized for its antimicrobial properties for centuries. Most studies on the antibacterial efficacy of silver, with particular emphasis on wound healing, have been performed on planktonic bacteria. Our recent studies, however, strongly suggest that colonization of wounds involves bacteria in both the planktonic and biofilm modes of growth. The action of silver on mature in vitro biofilms of Pseudomonas aeruginosa, a primary pathogen of chronic infected wounds, was investigated. The results show that silver is very effective against mature biofilms of P. aeruginosa, but that the silver concentration is important. A concentration of 5-10 mug/mL silver sulfadiazine eradicated the biofilm whereas a lower concentration (1 mug/mL) had no effect. The bactericidal concentration of silver required to eradicate the bacterial biofilm was 10-100 times higher than that used to eradicate planktonic bacteria. These observations strongly indicate that the concentration of silver in currently available wound dressings is much too low for treatment of chronic biofilm wounds. It is suggested that clinicians and manufacturers of the said wound dressings consider whether they are treating wounds primarily colonized either by biofilm-forming or planktonic bacteria.

Original language	English
Journal	Acta Pathologica Microbiologica et Immunologica Scandinavica
Volume	115
Issue number	8
Pages (from-to)	921-8
Number of pages	8
ISSN	0903-4641
DOIs	https://doi.org/10.1111/j.1600-0463.2007.apm_646.x
Publication status	Published - 2007

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@article{e0082cf0daa711df825b000ea68e967b,

title = "Silver against Pseudomonas aeruginosa biofilms",

abstract = "Silver has been recognized for its antimicrobial properties for centuries. Most studies on the antibacterial efficacy of silver, with particular emphasis on wound healing, have been performed on planktonic bacteria. Our recent studies, however, strongly suggest that colonization of wounds involves bacteria in both the planktonic and biofilm modes of growth. The action of silver on mature in vitro biofilms of Pseudomonas aeruginosa, a primary pathogen of chronic infected wounds, was investigated. The results show that silver is very effective against mature biofilms of P. aeruginosa, but that the silver concentration is important. A concentration of 5-10 mug/mL silver sulfadiazine eradicated the biofilm whereas a lower concentration (1 mug/mL) had no effect. The bactericidal concentration of silver required to eradicate the bacterial biofilm was 10-100 times higher than that used to eradicate planktonic bacteria. These observations strongly indicate that the concentration of silver in currently available wound dressings is much too low for treatment of chronic biofilm wounds. It is suggested that clinicians and manufacturers of the said wound dressings consider whether they are treating wounds primarily colonized either by biofilm-forming or planktonic bacteria.",

author = "Thomas Bjarnsholt and Klaus Kirketerp-M{\o}ller and S{\o}ren Kristiansen and Richard Phipps and Nielsen, {Anne Kirstine} and Jensen, {Peter {\O}strup} and Niels H{\o}iby and Michael Givskov",

note = "Keywords: Biofilms; Pseudomonas aeruginosa; Quorum Sensing; Silver; Silver Sulfadiazine",

year = "2007",

doi = "10.1111/j.1600-0463.2007.apm_646.x",

language = "English",

volume = "115",

pages = "921--8",

journal = "APMIS. Supplementum",

issn = "0903-465X",

publisher = "Wiley Online",

number = "8",

}

TY - JOUR

T1 - Silver against Pseudomonas aeruginosa biofilms

AU - Bjarnsholt, Thomas

AU - Kirketerp-Møller, Klaus

AU - Kristiansen, Søren

AU - Phipps, Richard

AU - Nielsen, Anne Kirstine

AU - Jensen, Peter Østrup

AU - Høiby, Niels

AU - Givskov, Michael

N1 - Keywords: Biofilms; Pseudomonas aeruginosa; Quorum Sensing; Silver; Silver Sulfadiazine

PY - 2007

Y1 - 2007

N2 - Silver has been recognized for its antimicrobial properties for centuries. Most studies on the antibacterial efficacy of silver, with particular emphasis on wound healing, have been performed on planktonic bacteria. Our recent studies, however, strongly suggest that colonization of wounds involves bacteria in both the planktonic and biofilm modes of growth. The action of silver on mature in vitro biofilms of Pseudomonas aeruginosa, a primary pathogen of chronic infected wounds, was investigated. The results show that silver is very effective against mature biofilms of P. aeruginosa, but that the silver concentration is important. A concentration of 5-10 mug/mL silver sulfadiazine eradicated the biofilm whereas a lower concentration (1 mug/mL) had no effect. The bactericidal concentration of silver required to eradicate the bacterial biofilm was 10-100 times higher than that used to eradicate planktonic bacteria. These observations strongly indicate that the concentration of silver in currently available wound dressings is much too low for treatment of chronic biofilm wounds. It is suggested that clinicians and manufacturers of the said wound dressings consider whether they are treating wounds primarily colonized either by biofilm-forming or planktonic bacteria.

AB - Silver has been recognized for its antimicrobial properties for centuries. Most studies on the antibacterial efficacy of silver, with particular emphasis on wound healing, have been performed on planktonic bacteria. Our recent studies, however, strongly suggest that colonization of wounds involves bacteria in both the planktonic and biofilm modes of growth. The action of silver on mature in vitro biofilms of Pseudomonas aeruginosa, a primary pathogen of chronic infected wounds, was investigated. The results show that silver is very effective against mature biofilms of P. aeruginosa, but that the silver concentration is important. A concentration of 5-10 mug/mL silver sulfadiazine eradicated the biofilm whereas a lower concentration (1 mug/mL) had no effect. The bactericidal concentration of silver required to eradicate the bacterial biofilm was 10-100 times higher than that used to eradicate planktonic bacteria. These observations strongly indicate that the concentration of silver in currently available wound dressings is much too low for treatment of chronic biofilm wounds. It is suggested that clinicians and manufacturers of the said wound dressings consider whether they are treating wounds primarily colonized either by biofilm-forming or planktonic bacteria.

U2 - 10.1111/j.1600-0463.2007.apm_646.x

DO - 10.1111/j.1600-0463.2007.apm_646.x

M3 - Journal article

C2 - 17696948

SN - 0903-465X

VL - 115

SP - 921

EP - 928

JO - APMIS. Supplementum

JF - APMIS. Supplementum

IS - 8

ER -

Silver against Pseudomonas aeruginosa biofilms

Abstract

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