Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast

Jelena Pavlovic; Jelena Samardzic; Vuk Maksimović; Gordana Timotijevic; Nenad Stevic; Kristian Holst Laursen; Thomas Hesselhøj Hansen; Søren Husted; Jan Kofod Schjørring; Yongchao Liang; Miroslav Nikolic

doi:10.1111/nph.12213

Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast

Jelena Pavlovic, Jelena Samardzic, Vuk Maksimović, Gordana Timotijevic, Nenad Stevic, Kristian Holst Laursen, Thomas Hesselhøj Hansen, Søren Husted, Jan Kofod Schjørring, Yongchao Liang, Miroslav Nikolic

Section for Plant and Soil Sciences

113 Citations (Scopus)

Abstract

Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.

Original language	English
Journal	New Phytologist
Volume	198
Issue number	4
Pages (from-to)	1096-1107
Number of pages	12
ISSN	0028-646X
DOIs	https://doi.org/10.1111/nph.12213
Publication status	Published - Jun 2013

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title = "Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast",

abstract = "Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.",

author = "Jelena Pavlovic and Jelena Samardzic and Vuk Maksimovi{\'c} and Gordana Timotijevic and Nenad Stevic and Laursen, {Kristian Holst} and Hansen, {Thomas Hesselh{\o}j} and S{\o}ren Husted and Schj{\o}rring, {Jan Kofod} and Yongchao Liang and Miroslav Nikolic",

year = "2013",

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doi = "10.1111/nph.12213",

language = "English",

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T1 - Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast

AU - Pavlovic, Jelena

AU - Samardzic, Jelena

AU - Maksimović, Vuk

AU - Timotijevic, Gordana

AU - Stevic, Nenad

AU - Laursen, Kristian Holst

AU - Hansen, Thomas Hesselhøj

AU - Husted, Søren

AU - Schjørring, Jan Kofod

AU - Liang, Yongchao

AU - Nikolic, Miroslav

PY - 2013/6

Y1 - 2013/6

N2 - Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.

AB - Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.

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SP - 1096

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Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast

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