Abstract
Organisms classified with the taxon 2 and taxon 3 complex of Pasteurellaceae have been obtained from lesions in ducks, geese, pigeons, partridges, pheasants and psittacine birds, but can also be isolated from apparently normal birds. We have investigated if biovars of taxon 3 dominated by pigeon isolates belong to
the same species, to outline an unambiguous diagnostic test, and to examine if differences exist between isolates form apparently healthy pigeons and isolates from lesions. A total of 76 isolates from pigeons (n=678) and other birds representing biovars of taxon 3 of Bisgaard were characterized genetically by partial
sequencing of the rpoB gene. A monophyletic group including the reference strain of Gallibacterium genomospecies 3 was formed with only up to 2% DNA sequence variation between strains. The group showed from 86 to 87% rpoB gene sequence similarity to the type strain of the type species of genus Gallibacterium (G. anatis). Five strains were classified with other taxa including two strains from pigeons related to the type strain of Pasteurella multocida. A single strain from a parakeet was related to members of the genus Volucribacter while a strain from a partridge showed identical sequence to the reference strain of taxon 14 of Bisgaard. Another strain from a budgerigar was closely related to the reference strain of taxon 44 of Bisgaard. In conclusion, the comparison of partial
rpoB sequences obtained from taxon 3 isolates of different biovars obtained from pigeons showed that these strains with two exceptions formed a homogenous genotypic group. Differences were not observed between isolates from lesions and apparently healthy pigeons
the same species, to outline an unambiguous diagnostic test, and to examine if differences exist between isolates form apparently healthy pigeons and isolates from lesions. A total of 76 isolates from pigeons (n=678) and other birds representing biovars of taxon 3 of Bisgaard were characterized genetically by partial
sequencing of the rpoB gene. A monophyletic group including the reference strain of Gallibacterium genomospecies 3 was formed with only up to 2% DNA sequence variation between strains. The group showed from 86 to 87% rpoB gene sequence similarity to the type strain of the type species of genus Gallibacterium (G. anatis). Five strains were classified with other taxa including two strains from pigeons related to the type strain of Pasteurella multocida. A single strain from a parakeet was related to members of the genus Volucribacter while a strain from a partridge showed identical sequence to the reference strain of taxon 14 of Bisgaard. Another strain from a budgerigar was closely related to the reference strain of taxon 44 of Bisgaard. In conclusion, the comparison of partial
rpoB sequences obtained from taxon 3 isolates of different biovars obtained from pigeons showed that these strains with two exceptions formed a homogenous genotypic group. Differences were not observed between isolates from lesions and apparently healthy pigeons
| Original language | English |
|---|---|
| Journal | Berliner und Munchener Tierarztliche Wochenschrift |
| Volume | 132 |
| Issue number | 7-8 |
| Pages (from-to) | 360-366 |
| ISSN | 0005-9366 |
| DOIs | |
| Publication status | Published - 1 Jul 2019 |
Keywords
- Sepsis
- phylogeny
- diagnostics