Serum from NSAID-treated patients attenuates the capacity of rat leukocytes to synthesize leukotriene B4

H. Gutheil; O. H. Nielsen; T. M. Hansen; B. S. Thomsen; I. Ahnfelt-Rønne

doi:10.1007/BF02263502

Serum from NSAID-treated patients attenuates the capacity of rat leukocytes to synthesize leukotriene B₄

H. Gutheil, O. H. Nielsen, T. M. Hansen, B. S. Thomsen, I. Ahnfelt-Rønne^*

^*Corresponding author for this work

Abstract

The synthesis of leukotriene B₄ by A23187-stimulated rat peritoneal leukocytes was studied in the presence of 0.1% normal human serum, serum from patients treated with NSAIDs for either an inflammatory (rheumatoid arthritis, RA) or a non-inflammatory condition (lumbar disc protrusion, LDP), and serum from RA patients drawn one week after withdrawal from NSAID treatment. The capacity for LTB₄ synthesis was significantly lower in the presence of serum from NSAID treated patients: thirty per cent less than observed in presence of normal serum in the RA group, and fifty per cent in the LDP group. When NSAIDs were withdrawn from RA patients, the LTB₄ production in presence of serum increased, but was not completely normalized after one week. These results indicate that NSAID treatment may down-regulate the capacity for leukotriene synthesis by an indirect effect.

Original language	English
Journal	Inflammation Research
Volume	45
Issue number	1
Pages (from-to)	31-34
Number of pages	4
ISSN	1023-3830
DOIs	https://doi.org/10.1007/BF02263502
Publication status	Published - 5 Feb 1996

Keywords

Leukotriene
NSAID
Prostaglandin
Rheumatoid arthritis
Serum

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10.1007/BF02263502

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title = "Serum from NSAID-treated patients attenuates the capacity of rat leukocytes to synthesize leukotriene B4",

abstract = "The synthesis of leukotriene B4 by A23187-stimulated rat peritoneal leukocytes was studied in the presence of 0.1% normal human serum, serum from patients treated with NSAIDs for either an inflammatory (rheumatoid arthritis, RA) or a non-inflammatory condition (lumbar disc protrusion, LDP), and serum from RA patients drawn one week after withdrawal from NSAID treatment. The capacity for LTB4 synthesis was significantly lower in the presence of serum from NSAID treated patients: thirty per cent less than observed in presence of normal serum in the RA group, and fifty per cent in the LDP group. When NSAIDs were withdrawn from RA patients, the LTB4 production in presence of serum increased, but was not completely normalized after one week. These results indicate that NSAID treatment may down-regulate the capacity for leukotriene synthesis by an indirect effect.",

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AU - Gutheil, H.

AU - Nielsen, O. H.

AU - Hansen, T. M.

AU - Thomsen, B. S.

AU - Ahnfelt-Rønne, I.

PY - 1996/2/5

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N2 - The synthesis of leukotriene B4 by A23187-stimulated rat peritoneal leukocytes was studied in the presence of 0.1% normal human serum, serum from patients treated with NSAIDs for either an inflammatory (rheumatoid arthritis, RA) or a non-inflammatory condition (lumbar disc protrusion, LDP), and serum from RA patients drawn one week after withdrawal from NSAID treatment. The capacity for LTB4 synthesis was significantly lower in the presence of serum from NSAID treated patients: thirty per cent less than observed in presence of normal serum in the RA group, and fifty per cent in the LDP group. When NSAIDs were withdrawn from RA patients, the LTB4 production in presence of serum increased, but was not completely normalized after one week. These results indicate that NSAID treatment may down-regulate the capacity for leukotriene synthesis by an indirect effect.

AB - The synthesis of leukotriene B4 by A23187-stimulated rat peritoneal leukocytes was studied in the presence of 0.1% normal human serum, serum from patients treated with NSAIDs for either an inflammatory (rheumatoid arthritis, RA) or a non-inflammatory condition (lumbar disc protrusion, LDP), and serum from RA patients drawn one week after withdrawal from NSAID treatment. The capacity for LTB4 synthesis was significantly lower in the presence of serum from NSAID treated patients: thirty per cent less than observed in presence of normal serum in the RA group, and fifty per cent in the LDP group. When NSAIDs were withdrawn from RA patients, the LTB4 production in presence of serum increased, but was not completely normalized after one week. These results indicate that NSAID treatment may down-regulate the capacity for leukotriene synthesis by an indirect effect.

KW - Leukotriene

KW - NSAID

KW - Prostaglandin

KW - Rheumatoid arthritis

KW - Serum

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Serum from NSAID-treated patients attenuates the capacity of rat leukocytes to synthesize leukotriene B₄

Abstract

Keywords

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