TY - JOUR
T1 - Serum Anticytokine Autoantibody Levels Are Not Increased in Hidradenitis Suppurativa
T2 - A Case-Control Pilot Study
AU - Theut Riis, Peter
AU - Von Stemann, Jakob Hjorth
AU - Kjærsgaard Andersen, Rune
AU - Hansen, Morten Bagge
AU - Jemec, Gregor B.E.
PY - 2017
Y1 - 2017
N2 - Background: Hidradenitis suppurativa is a skin disease of recurrent episodes of inflammatory nodules, abscesses, and scarring of the intertriginous regions, e.g. the axillae and groin. A dysregulated immune response to one or more unknown antigens in hidradenitis suppurativa has been suggested. One hypothetical element of this dysregulation may be the functionality of the cytokines. This study examines the serum level of anticytokine autoantibodies for interleukin (IL)-1α, IL-6, IL-10, IL-17A, IL-17E, IL-17F, and interferon-α. Method: Recombinant, carrier-free cytokines were coupled to microspheres. The coupled beads were incubated for 1 h in the dark with assay buffer-diluted sera, and subsequently for 30 min with polyclonal goat F(ab′)2 anti-human IgG phycoerythrin-conjugated antibody. Data are presented as the median fluorescence intensity of samples. Results: No difference in levels of anticytokine autoantibodies was demonstrated for any of the autoantibodies studied. Discussion: The data suggest that endogenously produced autoantibodies only play a minor role, if any, in hidradenitis suppurativa.
AB - Background: Hidradenitis suppurativa is a skin disease of recurrent episodes of inflammatory nodules, abscesses, and scarring of the intertriginous regions, e.g. the axillae and groin. A dysregulated immune response to one or more unknown antigens in hidradenitis suppurativa has been suggested. One hypothetical element of this dysregulation may be the functionality of the cytokines. This study examines the serum level of anticytokine autoantibodies for interleukin (IL)-1α, IL-6, IL-10, IL-17A, IL-17E, IL-17F, and interferon-α. Method: Recombinant, carrier-free cytokines were coupled to microspheres. The coupled beads were incubated for 1 h in the dark with assay buffer-diluted sera, and subsequently for 30 min with polyclonal goat F(ab′)2 anti-human IgG phycoerythrin-conjugated antibody. Data are presented as the median fluorescence intensity of samples. Results: No difference in levels of anticytokine autoantibodies was demonstrated for any of the autoantibodies studied. Discussion: The data suggest that endogenously produced autoantibodies only play a minor role, if any, in hidradenitis suppurativa.
KW - Acne inversa
KW - Anticytokine autoantibodies
KW - Cytokines
KW - Hidradenitis suppurativa
U2 - 10.1159/000475925
DO - 10.1159/000475925
M3 - Journal article
C2 - 28675899
AN - SCOPUS:85021952636
SN - 1018-8665
VL - 233
SP - 126
EP - 128
JO - Dermatology
JF - Dermatology
IS - 2-3
ER -
