Sequence and chromosomal localization of the mouse brevican gene.

TY - JOUR

T1 - Sequence and chromosomal localization of the mouse brevican gene.

AU - Rauch, U

AU - Meyer, H

AU - Brakebusch, C

AU - Seidenbecher, C

AU - Gundelfinger, E D

AU - Beier, D R

AU - Fässler, R

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; Brain; Chromosome Mapping; Cloning, Molecular; DNA Primers; Exons; Introns; Lectins, C-Type; Mice; Microsatellite Repeats; Molecular Sequence Data; Nerve Tissue Proteins; Polymorphism, Single-Stranded Conformational; Proteochondroitin Sulfates; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transcription, Genetic

PY - 1997

Y1 - 1997

N2 - Brevican is a brain-specific proteoglycan belonging to the aggrecan family. Phage clones containing the complete mouse brevican open reading frame of 2649 bp and the complete 3'-untranslated region of 341 bp were isolated from a mouse brain cDNA library, and cosmid clones containing the mouse brevican gene were isolated from a genomic library using a PCR-generated DNA fragment as probe. The obtained genomic sequence of 13,700 nucleotides revealed that the murine gene has a size of approximately 13 kb and contains the sequence of the mRNA for the secreted brevican isoform on 14 exons. The exon-intron structure reflected the structural organization of the multidomain protein brevican. No consensus TATA sequence was found upstream of the first exon, and RNase protection experiments revealed multiple transcriptional start sites for the brevican gene. The first part of the sequence of intron 8 corresponded to an alternative brevican cDNA, coding for a GPI-linked isoform. Single strand conformation polymorphism analysis mapped the brevican gene (Bcan) to chromosome 3 between the microsatellite markers D3Mit22 and D3Mit11.

AB - Brevican is a brain-specific proteoglycan belonging to the aggrecan family. Phage clones containing the complete mouse brevican open reading frame of 2649 bp and the complete 3'-untranslated region of 341 bp were isolated from a mouse brain cDNA library, and cosmid clones containing the mouse brevican gene were isolated from a genomic library using a PCR-generated DNA fragment as probe. The obtained genomic sequence of 13,700 nucleotides revealed that the murine gene has a size of approximately 13 kb and contains the sequence of the mRNA for the secreted brevican isoform on 14 exons. The exon-intron structure reflected the structural organization of the multidomain protein brevican. No consensus TATA sequence was found upstream of the first exon, and RNase protection experiments revealed multiple transcriptional start sites for the brevican gene. The first part of the sequence of intron 8 corresponded to an alternative brevican cDNA, coding for a GPI-linked isoform. Single strand conformation polymorphism analysis mapped the brevican gene (Bcan) to chromosome 3 between the microsatellite markers D3Mit22 and D3Mit11.

U2 - 10.1006/geno.1997.4853

DO - 10.1006/geno.1997.4853

M3 - Journal article

C2 - 9286696

SN - 0888-7543

VL - 44

SP - 15

EP - 21

JO - Genomics

JF - Genomics

IS - 1

ER -