Selective inbreeding does not increase gut microbiota similarity in BALB/c mice

Wanyong Pang; Damiano Stradiotto; Lukasz Krych; Peter Karlskov-Mortensen; Finn Kvist Vogensen; Dennis Sandris Nielsen; Merete Fredholm; Axel Jacob Kornerup Hansen

doi:10.1258/la.2012.012040

Selective inbreeding does not increase gut microbiota similarity in BALB/c mice

Wanyong Pang, Damiano Stradiotto, Lukasz Krych, Peter Karlskov-Mortensen, Finn Kvist Vogensen, Dennis Sandris Nielsen, Merete Fredholm, Axel Jacob Kornerup Hansen

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Abstract

Inflammatory diseases in mouse models are under strong impact from the gut microbiota. Therefore increased interindividual gut microbiota similarity may be seen as a way to reduce group sizes in mouse experiments. The composition of the gut microbiota is to a high extent defined by genetics, and it is known that selecting siblings as mothers even in inbred colonies may increase the gut microbiota similarity among the mice with 3-4%. We therefore hypothesized that selective breeding of mice aiming at a high similarity in the gut microbiota would increase the interindividual similarity of the gut microbiota. BALB/cCrl mice were, however, found to have a mean heterozygosity of only 0.8% in their genome, and selection of breeders with a high similarity in the gut microbiota for three generations did not change the overall gut microbiota similarity, which was 66% in the P generation and 66%, 64% and 63% in the F1, F2 and F3 generations, respectively. Increased gut microbiota similarity in closely related mice in inbred mouse colonies is, therefore, more likely to be caused by other factors, such as imprinting or different intrauterine conditions, rather than by residual heterozygosity.

Original language	English
Journal	Laboratory Animals
Volume	46
Issue number	4
Pages (from-to)	335-337
Number of pages	3
ISSN	0023-6772
DOIs	https://doi.org/10.1258/la.2012.012040
Publication status	Published - 2012

Keywords

Laboratory animal

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title = "Selective inbreeding does not increase gut microbiota similarity in BALB/c mice",

abstract = "Inflammatory diseases in mouse models are under strong impact from the gut microbiota. Therefore increased interindividual gut microbiota similarity may be seen as a way to reduce group sizes in mouse experiments. The composition of the gut microbiota is to a high extent defined by genetics, and it is known that selecting siblings as mothers even in inbred colonies may increase the gut microbiota similarity among the mice with 3-4%. We therefore hypothesized that selective breeding of mice aiming at a high similarity in the gut microbiota would increase the interindividual similarity of the gut microbiota. BALB/cCrl mice were, however, found to have a mean heterozygosity of only 0.8% in their genome, and selection of breeders with a high similarity in the gut microbiota for three generations did not change the overall gut microbiota similarity, which was 66% in the P generation and 66%, 64% and 63% in the F1, F2 and F3 generations, respectively. Increased gut microbiota similarity in closely related mice in inbred mouse colonies is, therefore, more likely to be caused by other factors, such as imprinting or different intrauterine conditions, rather than by residual heterozygosity.",

keywords = "Laboratory animal, Genetics, reduction, microorganism, rodents",

author = "Wanyong Pang and Damiano Stradiotto and Lukasz Krych and Peter Karlskov-Mortensen and Vogensen, {Finn Kvist} and Nielsen, {Dennis Sandris} and Merete Fredholm and Hansen, {Axel Jacob Kornerup}",

year = "2012",

doi = "10.1258/la.2012.012040",

language = "English",

volume = "46",

pages = "335--337",

journal = "Laboratory Animals",

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T1 - Selective inbreeding does not increase gut microbiota similarity in BALB/c mice

AU - Pang, Wanyong

AU - Stradiotto, Damiano

AU - Krych, Lukasz

AU - Karlskov-Mortensen, Peter

AU - Vogensen, Finn Kvist

AU - Nielsen, Dennis Sandris

AU - Fredholm, Merete

AU - Hansen, Axel Jacob Kornerup

PY - 2012

Y1 - 2012

N2 - Inflammatory diseases in mouse models are under strong impact from the gut microbiota. Therefore increased interindividual gut microbiota similarity may be seen as a way to reduce group sizes in mouse experiments. The composition of the gut microbiota is to a high extent defined by genetics, and it is known that selecting siblings as mothers even in inbred colonies may increase the gut microbiota similarity among the mice with 3-4%. We therefore hypothesized that selective breeding of mice aiming at a high similarity in the gut microbiota would increase the interindividual similarity of the gut microbiota. BALB/cCrl mice were, however, found to have a mean heterozygosity of only 0.8% in their genome, and selection of breeders with a high similarity in the gut microbiota for three generations did not change the overall gut microbiota similarity, which was 66% in the P generation and 66%, 64% and 63% in the F1, F2 and F3 generations, respectively. Increased gut microbiota similarity in closely related mice in inbred mouse colonies is, therefore, more likely to be caused by other factors, such as imprinting or different intrauterine conditions, rather than by residual heterozygosity.

AB - Inflammatory diseases in mouse models are under strong impact from the gut microbiota. Therefore increased interindividual gut microbiota similarity may be seen as a way to reduce group sizes in mouse experiments. The composition of the gut microbiota is to a high extent defined by genetics, and it is known that selecting siblings as mothers even in inbred colonies may increase the gut microbiota similarity among the mice with 3-4%. We therefore hypothesized that selective breeding of mice aiming at a high similarity in the gut microbiota would increase the interindividual similarity of the gut microbiota. BALB/cCrl mice were, however, found to have a mean heterozygosity of only 0.8% in their genome, and selection of breeders with a high similarity in the gut microbiota for three generations did not change the overall gut microbiota similarity, which was 66% in the P generation and 66%, 64% and 63% in the F1, F2 and F3 generations, respectively. Increased gut microbiota similarity in closely related mice in inbred mouse colonies is, therefore, more likely to be caused by other factors, such as imprinting or different intrauterine conditions, rather than by residual heterozygosity.

KW - Laboratory animal

KW - Genetics

KW - reduction

KW - microorganism

KW - rodents

U2 - 10.1258/la.2012.012040

DO - 10.1258/la.2012.012040

M3 - Journal article

C2 - 23097567

SN - 0023-6772

VL - 46

SP - 335

EP - 337

JO - Laboratory Animals

JF - Laboratory Animals

IS - 4

ER -

Selective inbreeding does not increase gut microbiota similarity in BALB/c mice

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Keywords

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