Selection of antimalarial drug resistance after intermittent preventive treatment of infants and children (IPTi/c) in Senegal

TY - JOUR

T1 - Selection of antimalarial drug resistance after intermittent preventive treatment of infants and children (IPTi/c) in Senegal

AU - Ndiaye, Magatte

AU - Tine, Roger

AU - Faye, Babacar

AU - Ndiaye, Jean L

AU - Diouf, Ibrahima

AU - Lo, Aminata C

AU - Sylla, Khadime

AU - Dieng, Yemou

AU - Hallett, Rachel

AU - Alifrangis, Michael

AU - Gaye, Oumar

N1 - Copyright © 2013 Académie des sciences. Published by Elsevier SAS. All rights reserved.

PY - 2013/5

Y1 - 2013/5

N2 - Senegal has since 2003 used sulphadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment (IPT) of malaria in risk groups. However, the large-scale IPT strategy may result in increasing drug resistance. Our study investigated the possible impact of SP-IPT given to infants and children on the prevalence of SP-resistant haplotypes in the Plasmodium falciparum genes Pfdhfr and Pfdhps, comparing sites with and without IPTi/c. P. falciparum positives samples (n=352) were collected from children under 5years of age during two cross-sectional surveys in 2010 and 2011 in three health districts (two on IPTi/c and one without IPTi/c intervention) located in the southern part of Senegal. The prevalence of SP-resistance-related haplotypes in Pfdhfr and Pfdhps was determined by nested PCR followed by sequence-specific oligonucleotide probe (SSOP)-ELISA. The prevalence of the Pfdhfr double mutant haplotypes (CNRN and CICN) was stable between years at

AB - Senegal has since 2003 used sulphadoxine-pyrimethamine (SP) for Intermittent Preventive Treatment (IPT) of malaria in risk groups. However, the large-scale IPT strategy may result in increasing drug resistance. Our study investigated the possible impact of SP-IPT given to infants and children on the prevalence of SP-resistant haplotypes in the Plasmodium falciparum genes Pfdhfr and Pfdhps, comparing sites with and without IPTi/c. P. falciparum positives samples (n=352) were collected from children under 5years of age during two cross-sectional surveys in 2010 and 2011 in three health districts (two on IPTi/c and one without IPTi/c intervention) located in the southern part of Senegal. The prevalence of SP-resistance-related haplotypes in Pfdhfr and Pfdhps was determined by nested PCR followed by sequence-specific oligonucleotide probe (SSOP)-ELISA. The prevalence of the Pfdhfr double mutant haplotypes (CNRN and CICN) was stable between years at

U2 - 10.1016/j.crvi.2013.04.016

DO - 10.1016/j.crvi.2013.04.016

M3 - Journal article

C2 - 23916206

SN - 0764-4469

VL - 336

SP - 295

EP - 300

JO - Comptes Rendus - Academie des Sciences, Serie III: Sciences de la Vie

JF - Comptes Rendus - Academie des Sciences, Serie III: Sciences de la Vie

IS - 5-6

ER -